FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq_1409_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq_1409_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099475
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG35190.1676133319044047No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA30960.14746543778801843No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT30330.14446468760047154No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG28600.13622453232355708No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC28480.13565296085926243No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA27500.1309851272341895No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG26050.12407863870729588No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC25400.12098262660903321No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA24810.11817240024291786No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG24600.11717215018040225No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT23640.11259957846604508No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC23120.11012276878743495No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT21980.10469283987663582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)