FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n02_ReSeq2_1794_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n02_ReSeq2_1794_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603021
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC36330.6024665807658439No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC24820.4115942894194398No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG18270.3029745232753088No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT17650.2926929576250247No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG16800.2785972627818932No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA13420.22254614681744087No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA12720.21093792753486196No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT12590.20878211538238306No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA12520.20762129345412514No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC12380.20529964959760935No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT12350.20480215448549885No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG11720.19435475713117786No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT11180.18539984511318844No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT11120.18440485488896738No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT10960.18175154762437792No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT10900.1807565574001569No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA10870.18025906228804636No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT10310.17097248686198324No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA10250.1699774966377622No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG10220.16948000152565168No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA10050.1666608625570254No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT9550.1583692773551833No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT9230.1530626628260044No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT9150.15173600919370966No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA9040.1499118604493044No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC9010.14941436533719388No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA8900.14759021659278865No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA8870.14709272148067812No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT8550.14178610695149918No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG8480.14062528502324131No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT8330.1381378094626887No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT7700.1276904121083677No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT7660.12702708529222034No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA7500.12437377802763089No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG7180.11906716349845195No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT7130.11823800497826775No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA7090.11757467816212039No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT7080.11740884645808354No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC7040.11674551964193619No Hit
GTTGGAAGAATGGTTGGTGGAATTGGGAGGTTTTACGTACAGATGTGCAC6960.11541886600964146No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC6960.11541886600964146No Hit
ATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTC6950.11525303430560462No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT6840.11342888556119936No Hit
GCATTAACCAATCCCTTGACATTTCCTCAAGAGTCATTTCAGTTAGGTAG6840.11342888556119936No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC6810.11293139044908883No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAGAAAAGACG6760.11210223192890463No Hit
ATCAAGTGCAATATCCCAATGATACTTGCTGCAACAACGAGAGGATCACT6700.1111072417046836No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC6670.11060974659257305No Hit
TTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCATTAAC6630.10994641977642569No Hit
CTCATATACAACCGGATGGGAACAGTGACCACAGAAGTGGCTCTTGGCCT6590.10928309296027834No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA6580.1091172612562415No Hit
GTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCA6320.10480563695128363No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG6220.1031473199109152No Hit
ATCCCAATGATACTTGCTGCAACAACGAGAGGATCACTTGAATCTCTGCA6210.10298148820687837No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGG6200.10281565650284152No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTC6190.10264982479880468No Hit
GTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA6120.1014890028705468No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG6070.1006598443503626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)