FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_Blank_Well1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_Blank_Well1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGGTGTTTTATTATGGCCGCCTGAGCTAATATATCTGGTGTTTTG22.631578947368421No Hit
GATACTTTCTATAGGACTTCAAAGCTAGTTGGAATCAACATTAGAAAAAA11.3157894736842104No Hit
CTAAAGAACTACCAAGAAAGAAAAAATCATCATCACAGAGGCAGTAGGGA11.3157894736842104No Hit
GTTCCTCTCCAAGCACAGAGCCAAAGGCTGGGAACCATCCACGCCCCCTG11.3157894736842104No Hit
GTCTATCGAAATATACATGAGTTTAATAGGCATTAGAGTAATTGGGCAAC11.3157894736842104No Hit
GTCCTCAAGGTTAAGCCCCATCCAAGTTCACCCAGTAGCTAAGAACTATG11.3157894736842104No Hit
GGGTAGACTTCTTGGGTAGAGAGAGGCATTCTCTCACGGCCTGAAGGGTA11.3157894736842104No Hit
GGGTAGTCTAATGAAGCATAGAACATAGACAGTACGGGATTAAAAAAATT11.3157894736842104No Hit
TCTTTGAAGTCCTCAAACTCCAATTTTTCCGGTGCCAAATTCTCCCCCAC11.3157894736842104No Hit
GAAGAACTCTTTTCAGCATTTTATGCTGATAGGAAATTCCTGTTGAGCCT11.3157894736842104No Hit
GAAGGCAAGGTTTGGGAAACCATTGAAGTAGCCGTTCGGCAAGGTATCGA11.3157894736842104No Hit
ACCTACTAATATTGAATCATAAAGAAAAAGAAATATGAACAGACTAATGA11.3157894736842104No Hit
AAAGATGAGATCTTCAATTTCAGCATTCCCAGGATTTCTGCTTTCTCGCA11.3157894736842104No Hit
GGAGAAACAGGCTCCCCGCTGAGCAAAGAGCCCAATCTGGGGCTCGATCC11.3157894736842104No Hit
CTGATCTGCACTTCAATTATTTCACTGGCATACATCACCGATTGGCGGTC11.3157894736842104No Hit
ACTGCAGGGCTGCCTCGGGGAGGGTCAGTGGCATCGGGCTGCTTTGGCGC11.3157894736842104No Hit
GCCGCGAATTGTTAGTAGGATGGTGTTGACGTAAGGCGGAGCCCCAGGAG11.3157894736842104No Hit
GACATGGGTGTGCAGTGACACTATCAGATTAAACTCCAGTCAAACTGGAC11.3157894736842104No Hit
GCACCACCAGCAGGATGGACAAGACAAGGCGCCCTTGTGTTCGTCAGCTA11.3157894736842104No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG11.3157894736842104No Hit
CGCTTAGCCACTGAGCCATCCAGGTGCCCCTAAAAATAAGAACTCCTTTT11.3157894736842104No Hit
CCTTTCTGCTATGAGGCTGAATGCTGTGTTTGTGAATGTATGTGTCACTC11.3157894736842104No Hit
CTTTTGACTTCTGGCACATGGACCTGCTCAGTGGCAAACAAACCACCACC11.3157894736842104No Hit
CCACAAAGATGCACCTAACATAACATCTCCTTCTCTCCAAAACTTCCTTA11.3157894736842104No Hit
AGTTACCTGTATTAACTCCGAGGGCTCAGATTTCTTTTAAAATCGTGAAA11.3157894736842104No Hit
GGTGAAAGGCTGTGCCTAGCAGTGGTCCAATTTGCTGGCTTGACTCTCTG11.3157894736842104No Hit
CTGCAAAAACATACCAAATTGTAAAGAACATCAACACTATGAAGAAACTG11.3157894736842104No Hit
GTATGAAAGAATGCCGTATTACGCAGGACACTGAGGTCCTATTCTGTGGA11.3157894736842104No Hit
CATTTGAACTAACTGCCTCCTCCACCTGGAGGGCCAGAAACCCAGATCCC11.3157894736842104No Hit
TGCAGTACCTTGGGCAGGTGACTGCATCTGTGTTTCATCATCTGTAAAAT11.3157894736842104No Hit
AATAAAAAGTCTCCCAGAAAAGAAAAGCCTGGGACCCAATAGCTTCACTG11.3157894736842104No Hit
ACTCTAATCTGAATTTATTTTTCGAATGATCCCCATTGCTAGTGCCGTGA11.3157894736842104No Hit
GCGCAGCGGGGTGCCTGCTTCTCCCTCTCGTGCGCGGTCGTGCTCTCTGT11.3157894736842104No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA11.3157894736842104No Hit
GATACAGATGTGTGATGACACGAAACACTTCACACGAACTCGCTAACAAG11.3157894736842104No Hit
GTACAACTGTAGTACTGCTTATGCTTTCTAGACAGGTCCATGAATTAAGC11.3157894736842104No Hit
GGCCCAGGTGACAAACATGCAGGGAGACTAAACGGACATTTACTATCCTG11.3157894736842104No Hit
TTAATGTTGAGAGTCCAAAAGAGTTTCTCTGAAGGAGACAGGACGCAGCA11.3157894736842104No Hit
CATCCAAGTAAGGGGTTGTCTCAGGCTGATGTTTCCATTGTGATCTTCTG11.3157894736842104No Hit
GTACATGGTCTGAGGAACAGAGAAACACATAAATCCATGGAGGTGTATGA11.3157894736842104No Hit
GATCTCAATAGAGTTATTATTAAAACTCGGAGAAGTCACAGAAAGGAGAA11.3157894736842104No Hit
ATACATTTATAATCTGCCTCCCCGAATGGATTATGAAGCCCTGATACATG11.3157894736842104No Hit
TGATGGAATTGGGCTTATTAATGAACAGATACTGTAACTGTTCTTTATGT11.3157894736842104No Hit
GCTTTTGGATGGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAG11.3157894736842104No Hit
AGAACAATATCAGATCAGCAAAGAGCTGGTTCTTTGAAAAGCCCAAATCT11.3157894736842104No Hit
TTAATAAATAGAACATATTTAAGCAAATGTCATATGTTTCCTAAATAATG11.3157894736842104No Hit
CCCTTGGTCCCAGAATCAAAGTTAGATGCCCCCTTGCAGATACAGTGAGG11.3157894736842104No Hit
TAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCA11.3157894736842104No Hit
GTTCGACCCTGTGGAGCCAGTCTGCCTCAGGTCATCAAGGTTACTGGAGC11.3157894736842104No Hit
GGGAGAGAGAGACAGACAGACTGAGCAGCAGGCTGTAGAAGAGGGAGAAA11.3157894736842104No Hit
GAAAAGCGGACTCATCATCAGAAATGAAAGAGATGTTCCCTGAAACCACT11.3157894736842104No Hit
CATCTGTCGATGACAGGAGGATGGATCATCTTTCACTGGAGCAGGAAAAA11.3157894736842104No Hit
GTGTGTGTGTGATGGGCACCTGCTAGCAGAAGTGAGGGGATCTATAGTGT11.3157894736842104No Hit
GGGTTTCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTG11.3157894736842104No Hit
CCCTTAATAATGGTGTAAACCATAATAAAACTAATACTACTACATGATCA11.3157894736842104No Hit
CAAATAGGCAGAGAGACAGACAGAGAGAGAGATGAGGAAAAGGAGCCTCC11.3157894736842104No Hit
CTGTAGTTCATCTAAGCCACTTAATAAAATAAAAACGCGGGCTGTGTTTT11.3157894736842104No Hit
TTCCTGTGTGATCTAACTAAAATGATTACTGGGCTCTGGGTCACTCTACT11.3157894736842104No Hit
TCGCTGGCCCCAGGCAAGTCACCACCACAGACAGGGCCAGAAGGGAAAAT11.3157894736842104No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTACCCTTTTCTTTTGGATTA11.3157894736842104No Hit
GTGAAATATATATAAGCATAAATTATCCAGAATGCCAGAAGAGAAAAAGA11.3157894736842104No Hit
AACCAATTGGGTTCATGGGCAAACCAAAATAGGAACACAGAAATACTTTG11.3157894736842104No Hit
CCCCCCCACCCCCGCCATGGGGAGAACTCCACCCACCATAGAAACCGGAA11.3157894736842104No Hit
CTTCTGCCTTAGAGCATCTAAGCTAGTTATTGCCTCTGCTAGAAATGTTC11.3157894736842104No Hit
GTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTG11.3157894736842104No Hit
TTGACATGGTCCTATGATGTTGATCCTTCGTTGTGTGAATGTGGCACATT11.3157894736842104No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTAGTGCGACAGTGCTTCAATCC11.3157894736842104No Hit
GTCCCTGAGCCTCCCCCTGTGGGGGTTCAGACCCGCGGCAGCCCCAGCAA11.3157894736842104No Hit
TTAACAATCTTACAAGAATTTTGATTGAGACCACATTAATTTGCTATATT11.3157894736842104No Hit
CCTCCGCAAGCCCTGCTCTGGAAGTCAGTAGAAACACCTGCCCATGGTTT11.3157894736842104No Hit
TACACCACGTAAGTAGTACCTTCCACCATTAGCCGCTGCAACAATGAGTT11.3157894736842104No Hit
GTCCTTTTTAGAATTAAGAGGAAGTCTACGCCTCGAATAATCAACTAAAA11.3157894736842104No Hit
CTTTAATGGGTACAGTCCTGGATTTTTCTACCTATAAGGATGCATTATTA11.3157894736842104No Hit
GTATAATAGCATAACTTGCCTTCCGTTGTAATGATGTTTGACAGGTCAGT11.3157894736842104No Hit
ATGGTCCACACGTTATAATTCTAACTACCAGTCTAGAGTCCTTACTGTAT11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)