FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_2243_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_2243_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6589587
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG491010.7451301576259635No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG455270.6908930711439123No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA349150.5298511120651416No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC347590.5274837406350352No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT333920.5067388897058344No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC326890.4960705428124707No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT326280.49514483988146757No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT311560.4728065658743105No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT273130.41448728122111445No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG243730.3698714350383415No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC238130.3613731786225753No Hit
CTATTGCACCGTTCGTGACAGTAAAGATGTGGTAAACACCACTGAGCAGG236760.3592941408922896No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA236170.35839878887705706No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG213860.3245423423349597No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT200910.3048901243735002No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT198310.30094450532332295No Hit
GCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCG186070.28236974487171956No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT184240.2795926360787102No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA179510.2724146444989648No Hit
GAGTAATACAGTGCTATCAAGCTGTTCAAGTGCCATACTGACATACCCTG176870.2684083236172464No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA176020.2671184096969962No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG173120.26271752691026007No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA170380.2585594514496887No Hit
GAGCAAAAGCAGGTCGCGGCGTATATTTTGGCGGCTCAGATTTTGCCTGC164330.2493782994290841No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT158920.24116837671313848No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT157420.23889205803034394No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC153920.23358064777049No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT152740.23178994374002498No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC152720.2317595928242544No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG152100.23081871443536597No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA150670.22864862395776853No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC147580.22395940747121176No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT147000.22307923091386458No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG145600.22095466680992298No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC143600.21791957523286362No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG143200.21731255691745172No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT143080.21713045142282816No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT141600.21488448365580426No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG140480.21318483237265096No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG139830.21219842761010668No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG135490.2056122788878878No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG133280.2022585026952372No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC132320.20080165873824868No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA129950.1972050752194333No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC127190.1930166488430914No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA121490.18436663784847215No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG120850.18339540854381314No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT120140.18231795103395707No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC119820.18183233638162757No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT117930.17896417484130644No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG117910.17893382392553586No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA115680.17554969681711463No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC114730.17410802831801142No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG114720.17409285286012613No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA113980.17296986897661415No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA113820.1727270616504494No Hit
TTCCATAACTGTCTCAAAAGTGACCATAAGCTCTCCTGAATCAGGAGAGC113500.1722414469981199No Hit
GCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGC113160.1717254814300198No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG112570.17083012941478729No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG107810.16360661146138597No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT107290.1628174876513505No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC106750.1619980129255445No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC105780.1605259935106707No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG104910.15920572867464988No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT104640.15879599131174685No Hit
GAGCAAAAGCAGGTTCTGAAAGTGAGTATGCAAAGCAATGCACCTGTGCC102550.15562432061371978No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG102430.15544221511909623No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG101700.15433440669346954No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC99440.15090475321139246No Hit
CTATTGCACCGTTCGTGACACAGACTCGGGATCAATTTAGCTTTGTTGAG98890.15007010302770113No Hit
TTCCCTGCTTGTGCAGGATTTTAATTTCCATAACTGTCTCAAAAGTGACC98090.14885606639687737No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG97110.1473688715241183No Hit
GTACAAATGGATCAAGGTGAACAACTAAAGGAACTCTCTGGTGTTGCGAC96260.14607895760386805No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT96060.1457754484461621No Hit
CCTCTTACAGTCGGAAAAGTTACTTTTGCGCAGCGCCCATTTGGGTCACC95110.1443337799470589No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG94450.1433321997266293No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT92840.1408889510070965No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA92740.14073719642824353No Hit
GTATTACTCAGTCTGCTGCTGTTGTATGTTATGTCAGGCATTTTACTGAT92240.13997842353397869No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC91730.13920447518182855No Hit
AGCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC91530.1389009660241226No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT90090.13671570008863984No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA88680.13457596052681298No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC87360.1325728000859538No Hit
CTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTG86440.13117665796050648No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC85680.13002332316122392No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT85460.12968946308774737No Hit
GGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATT84500.12823261913075887No Hit
GAATACATATACTGGAACTGCATCAGTAAAATGCCTGACATAACATACAA83670.12697305612627924No Hit
GATATAACCTGTGGATACTGCCAAAAACTGCATCATGATCTGAAGTCTTA83320.12644191510029384No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG81820.1241655964174993No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA80990.12290603341301966No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC80380.12198033048201654No Hit
AGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC80360.12194997956624595No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT79900.1212519085035223No Hit
CCACTGAGCAGGCCATTTTAATGGGCGTCCGGGAATTCGCATCCAAAACA79250.12026550374097801No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA79160.12012892462001032No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT78960.1198254154623044No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC78320.11885418615764538No Hit
GTAGGTGTACAGCCCGTTTTCGCTGCACAATGTCAGTACGGAGCCTGCGG77840.11812576417915115No Hit
TGGTAGGAGTACCTTGCAGCGGGATTGTTGAGGCAACCTGGAAATGCGAT77830.11811058872126584No Hit
GTTCGTGACAGTAAAGATGTGGTAAACACCACTGAGCAGGCCATTTTAAT76950.11677514842735973No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG76940.11675997296947442No Hit
CTCTGGTGTTGCGACCACAGGTGACACCATCTCTAAAAAGATGGAAAGCT76730.11644128835388318No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG75670.1148326898180417No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG75020.11384628505549742No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT73800.11199487919349119No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT72820.11050768432073207No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG72680.11029522791033793No Hit
TACTGGAACTGCATCAGTAAAATGCCTGACATAACATACAACAGCAGCAG72470.10997654329474668No Hit
GTAAGAGGTGTGTATGAAATCTACTTTACTGAGTACGTTAATGCTGGTTG72230.10961233230549958No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG71860.10905084036374357No Hit
CTCCTGAATCAGGAGAGCAGATTACCCCCTGGATCTGATTTCAGGCGTTG71590.10864110300084057No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT71410.10836794475890524No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC70880.10756364549098449No Hit
GTGCAGGACTGAACTCGGTAGTAGCCGGGAACATGGCCAAAATCTGGTGT70770.10739671545424623No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC70750.10736636453847563No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC70360.10677452168094904No Hit
GCAAAAGCAGGTCGCGGCGTATATTTTGGCGGCTCAGATTTTGCCTGCAA69800.10592469603937242No Hit
TGTCAGTACGGAGCCTGCGGAACGGAAAACGATCCAGGAATCGTATTTCT68590.10408846563525151No Hit
ATGCAAAGCAATGCACCTGTGCCGTCAGACAGCATTGCTTATTCAGCAAT68430.10384565830908674No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT68220.1035269736934955No Hit
CCCTACGGCTCAGACGTTCATAACAACGTTTTATTACGGGATCCCGGAGA67960.10313241178847778No Hit
CCTGTGGATACTGCCAAAAACTGCATCATGATCTGAAGTCTTATCTTGAT67830.10293513083596893No Hit
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG67460.10237363889421296No Hit
GGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCG67110.10184249786822756No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA66920.10155416416840692No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG66370.10071951398471558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)