FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_1984_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_1984_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815373
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGGCTATGTATTGAGCACGCAGGAGTGGGTGCCTTTTAGGGGGAG15030.18433281455235825No Hit
GAACAGAGACAGACCATCAGCTCTCTGCCCACGAGGTTCCTTTCCAAAGG12190.1495021297001495No Hit
GAGTTAAAGGTTTGTAAAGGAAAATGGTTAAAAAAAGAGAGAGAAAGAAA11270.1382189501001382No Hit
GTATTGAGCACGCAGGAGTGGGTGCCTTTTAGGGGGAGGCCCCCAAGCGT10990.1347849389175261No Hit
TCCCTATCCAGAGCAGAGTTAGGCTTTGAATGGCAGGTGGGCCCCAGCCA10710.13135092773491397No Hit
GTCCATGTGTGGCCACGTGCTCAATACAGCCACCTCCACATGGAAAGCCT10450.12816220306534556No Hit
GAGCACCCCTGCCTATGTTGGGGCTTCCTGTCCAAGGCTATGTATTGAGC10050.12325647280447109No Hit
GTTCTAGGGCATGATTGCATGTTGGTTCATGAAAAGCGCACAGAGCACCC9420.11552994764359378No Hit
GTGTTACTCTGTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTT9390.1151620178740282No Hit
GCTATGTATTGAGCACGCAGGAGTGGGTGCCTTTTAGGGGGAGGCCCCCA9320.11430351507837518No Hit
GTATATGGAGAAAGCAGTTGTTTTGAGTCCTGGAGTTTTAGAGAAAATGG9100.11160536343489422No Hit
GTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTTTCAGTGCCAT8820.10817135225228208No Hit
CATCATATGATTAAATAAAGGTAATAAAGGCCATCAGAATTCCAGAATGG8270.10142597314357969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)