FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_1981_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_1981_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201052
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC129610.5888547839851125No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG68860.31285040062660946No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT54590.2480177660500524No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT45910.2085820780245083No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT45520.20681019803257716No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA44700.20308470676749119No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA41720.1895457263163251No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC39960.18154954994248207No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT39390.17895987918504425No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA37990.17259928434221455No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT35520.1613773777266507No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC34640.15737928953972918No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA32750.1487924865019091No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG31870.14479439831498755No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA29770.135253506050743No Hit
GGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGTAGTCG29590.13443571528523635No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT27090.12307751020875474No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT27060.12294121174783695No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG27020.12275948046661324No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA26450.12016980970917543No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA26050.11835249689693837No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC25640.11648975126439538No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA25500.11585369178011241No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA25000.1135820507648161No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC24830.11280969281961535No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG24730.11235536461655607No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG24280.11031088770278939No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT23950.10881160463269382No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGG23880.10849357489055234No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA23310.10590390413311453No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA23080.10485894926607821No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT22600.10267817389139375No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT22200.10086086107915669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)