FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_1971_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_1971_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96489
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAACTCAGCACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTT6720.6964524453564656No Hit
CCATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACCCTGGATC5060.5244121091523386No Hit
GTCCCAAGACTGTCTCCTGATAAGAGCTTTTTGCTGTGGTACCCCGAGGG4740.4912477069925069No Hit
CACATGGGGAGGTGGCCACCAAGGCTGATGGTGGCAGGACCAGTGCTGAG4250.4404647161852646No Hit
CCCCCGACTCGTGCAAGCCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCA4060.4207733524028646No Hit
GTCATGTATCATGCTAATTGAAATGATGGGCTATTCTTCTAGGTGTACAA3780.3917545005130118No Hit
ATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTGGCCCT3770.39071811294551717No Hit
GTGCTGAGCCCGATGAGCTTCTGGAGGCAGGAGGAGCAGGAGGCGGCCCT3590.37206313673061175No Hit
GCCCTGAGCAGAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCTGGCAG3540.3668811988931381No Hit
GCCTGGCCCTGAGCAGAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCT3300.3420078972732643No Hit
CCCTGGATCCTTGCCACTGTCAAAAGAAAAAGCACCCACAGAGGCAGGGT3270.33889873457078007No Hit
GAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACCCTGGATCCTTGC3210.33268040916581165No Hit
GCAATAGGCTTTGCTCTGAAAGTGCACTTGGGAGAGATTGGGTGGCACGT3150.3264620837608432No Hit
TCTTTATACTTTGACTTATTCAGTTAACAGAGAGGACTAAAGGTGACAGT2890.29951600700597997No Hit
GACCAGTGCTGAGCCCGATGAGCTTCTGGAGGCAGGAGGAGCAGGAGGCG2850.295370456736001No Hit
AGCCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCATGGACCAGAACTCAG2780.2881157437635378No Hit
GTACTAAACAAATTTAACCTAAGTAGTGAAATTTTGCAGTTTATGAGAAG2680.2777518680885904No Hit
GCCTGGTTCATGCCATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGG2670.2767154805210956No Hit
GAACTCAGCACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGT2620.27153354268362195No Hit
ACTCAGCACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGC2510.2601332794411798No Hit
GTGCAAGCCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCATGGACCAGAA2490.2580605043061904No Hit
CTCTGGGGTGGCGAGAGGGAAAGGGAGCTTCTGGCCAGGGATTGGTCTTG2360.24458746592875868No Hit
CCGTACAGCCGTCCTGAGGGGCATGCACCCTCGAAGGGGCCAACACTTGG2350.24355107836126397No Hit
GACTTATTCAGTTAACAGAGAGGACTAAAGGTGACAGTGATGGGGACTTT2340.24251469079376925No Hit
GCCAAGACCAATCCCTGGCCAGAAGCTCCCTTTCCCTCTCGCCACCCCAG2230.23111442755132708No Hit
CATGGGGAGGTGGCCACCAAGGCTGATGGTGGCAGGACCAGTGCTGAGCC2200.22800526484884287No Hit
GAGCAGAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCTGGCAGCAGAT2140.22178693944387443No Hit
GTGGTACCCCGAGGGGCTGCTGAGGGGGGGACCTTTGTGGGCTTTGCCAC2040.21142306376892703No Hit
GTCAAAAGAAAAAGCACCCACAGAGGCAGGGTCCCCATGCTGTGTCCCAA2020.20935028863393754No Hit
CCCCACACCGCAGCCCACTGCCGTGCTACAAGGGCAGGAGCGAGCATCCC2000.20727751349894807No Hit
GGGCAGGAGCGAGCATCCCACCGACCCCAGTGGTGCTCTCTCATCCTCTG1990.2062411259314533No Hit
GTCCTTGGCTGAGGCTTGCCCCTTACCGACTGGCTTTGGCACGCTGGGCG1960.2031319632289691No Hit
GTCCCCATGCTGTGTCCCAAGACTGTCTCCTGATAAGAGCTTTTTGCTGT1910.19795002539149542No Hit
GGGGAAGGCAGCGGGGATTCACATGGGGAGGTGGCCACCAAGGCTGATGG1840.1906953124190322No Hit
GTTCATGCCATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACC1800.18654976214905328No Hit
GTAATATGGATGGCTGCTGTGCAGCCTTAGATTTCCCTCTTGACTTCTGT1770.18344059944656904No Hit
GTTTATGAGAAGTGTGATTTTGATTACCTTGTTTCTGTAGATTCTGTTGT1770.18344059944656904No Hit
GTCTTGGGACACAGCATGGGGACCCTGCCTCTGTGGGTGCTTTTTCTTTT1730.1792950491765901No Hit
ACCCCAGAGTGGCCAGGCAGTGGCAAAGCCCACAAAGGTCCCCCCCTCAG1710.1772222740416006No Hit
GTGCTGGGGGAGGAATGCCGGTCTCAAAAGGCACTGATCCCGTGCTACCT1700.17618588647410585No Hit
TCTGGAGGCAGGAGGAGCAGGAGGCGGCCCTGCCTGGTTCATGCCATAGA1640.1699675610691374No Hit
GTAATAGACATCCTGGAACTCAAGCTCCTGCTCTCTGAATGTTATTAGTT1630.1689311735016427No Hit
GATACACCTCGCTCCTGCGGTTCCCACTCCCAACAGGATGGAGCTGCCAA1630.1689311735016427No Hit
CCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTGG1620.16789478593414794No Hit
GGATTATTTTTCTCAACTAAGTTCATCCAGACTAGACACACAAATATTGA1580.16374923566416896No Hit
GTGTACTATCAGAACTTTCATCCAGTTATTGTTTAACATTGCTTAAGTCT1570.16271284809667425No Hit
ACCTGCAGGGGGGCTGCAATAGGCTTTGCTCTGAAAGTGCACTTGGGAGA1570.16271284809667425No Hit
CCCCAGTGGTGCTCTCTCATCCTCTGGTGCTATCGACCAAAACCCAAGCA1560.1616764605291795No Hit
AAGCAGGGCCATGAAATGCTCCTTTTCAGGCAAGTAATATGGATGGCTGC1550.16064007296168475No Hit
CAGATACACCTCGCTCCTGCGGTTCCCACTCCCAACAGGATGGAGCTGCC1540.15960368539419No Hit
GCTGAGGGGGGGACCTTTGTGGGCTTTGCCACTGCCTGGCCACTCTGGGG1540.15960368539419No Hit
GTCATTTGCTATGTGTACTATCAGAACTTTCATCCAGTTATTGTTTAACA1540.15960368539419No Hit
CTACAGGATTATTTTTCTCAACTAAGTTCATCCAGACTAGACACACAAAT1530.15856729782669526No Hit
TATTAGAACCCTCTGGAAGGAAATATTTAAGTAACACTTTAGATGAAAAC1500.15545813512421106No Hit
CCTGGGGAGGGGCCGCGAAGGTGTTTTCCCCCATGGACCAGAACTCAGCA1490.1544217475567163No Hit
TATAGAAGCAGATCCAATATTTTCCAGGTAATAGACATCCTGGAACTCAA1410.14613064701675837No Hit
CCCCAGAGTGGCCAGGCAGTGGCAAAGCCCACAAAGGTCCCCCCCTCAGC1410.14613064701675837No Hit
GCCATGAAATGCTCCTTTTCAGGCAAGTAATATGGATGGCTGCTGTGCAG1400.14509425944926366No Hit
AGCGAAAGCAGGGCCATGAAATGCTCCTTTTCAGGCAAGTAATATGGATG1390.1440578718817689No Hit
CAGTTAACAGAGAGGACTAAAGGTGACAGTGATGGGGACTTTTGAGAAGC1390.1440578718817689No Hit
GTTCTCCTTCGAGTGCCAGCCTGGCCCTGAGCAGAGGCAACAGAGGCAGA1390.1440578718817689No Hit
ATAGAAGAGGGCTTTGCTTCTCAAGTCGCAGCTGGGAGACCCTGGATCCT1380.14302148431427417No Hit
GTTTTCCCCCATGGACCAGAACTCAGCACCTTATTCAAATCCTGTTGGAA1380.14302148431427417No Hit
CTACAAGGGCAGGAGCGAGCATCCCACCGACCCCAGTGGTGCTCTCTCAT1380.14302148431427417No Hit
TTTTAATGCTACAGGATTATTTTTCTCAACTAAGTTCATCCAGACTAGAC1380.14302148431427417No Hit
GAGGCAACAGAGGCAGAAAAATGCCAGGATGCCCTGGCAGCAGATACACC1370.14198509674677942No Hit
GTTAACAGAGAGGACTAAAGGTGACAGTGATGGGGACTTTTGAGAAGCAG1340.1388759340442952No Hit
GCCCACTGCCGTGCTACAAGGGCAGGAGCGAGCATCCCACCGACCCCAGT1340.1388759340442952No Hit
GTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTGGCCCTGAGCAGAGGCA1320.13680315890930572No Hit
ACCTTATTCAAATCCTGTTGGAAGCCAGTTCTCCTTCGAGTGCCAGCCTG1320.13680315890930572No Hit
GGATGGAGCTGCCAAGACCAATCCCTGGCCAGAAGCTCCCTTTCCCTCTC1270.13162122107183202No Hit
GGGTACCACAGCAAAAAGCTCTTATCAGGAGACAGTCTTGGGACACAGCA1260.13058483350433728No Hit
ATATTGAAGTGTTTGTAACAACAGAATCTACAGAAACAAGGTAATCAAAA1260.13058483350433728No Hit
AATTTAACCTAAGTAGTGAAATTTTGCAGTTTATGAGAAGTGTGATTTTG1250.12954844593684253No Hit
CTCCCAACAGGATGGAGCTGCCAAGACCAATCCCTGGCCAGAAGCTCCCT1250.12954844593684253No Hit
GACTAAAGGTGACAGTGATGGGGACTTTTGAGAAGCAGCTAAAAATCCCA1250.12954844593684253No Hit
CCCAAGCACTTTCCAAGAAAACCAAGCTGTTTTTCCGTCTAGTTTACACA1240.1285120583693478No Hit
CTCCTGATAAGAGCTTTTTGCTGTGGTACCCCGAGGGGCTGCTGAGGGGG1230.12747567080185307No Hit
GATTCACATGGGGAGGTGGCCACCAAGGCTGATGGTGGCAGGACCAGTGC1220.12643928323435832No Hit
GCCTTAGATTTCCCTCTTGACTTCTGTCTTGGCCAGCAGAATTCTGAGTG1200.12436650809936885No Hit
GACCAATCCCTGGCCAGAAGCTCCCTTTCCCTCTCGCCACCCCAGAGTGG1190.1233301205318741No Hit
CCCATGCTGTGTCCCAAGACTGTCTCCTGATAAGAGCTTTTTGCTGTGGT1180.12229373296437934No Hit
ATACATGACTTTGTGTTTTCATCTAAAGTGTTACTTAAATATTTCCTTCC1180.12229373296437934No Hit
CTCTGGAAGGAAATATTTAAGTAACACTTTAGATGAAAACACAAAGTCAT1180.12229373296437934No Hit
CCTTAAGCCTGACCACCACCCATCTGACGCCCTTGGTTTTGCAATACAAG1170.12125734539688462No Hit
ACTTTGACTTATTCAGTTAACAGAGAGGACTAAAGGTGACAGTGATGGGG1170.12125734539688462No Hit
CTCTCATCCTCTGGTGCTATCGACCAAAACCCAAGCACTTTCCAAGAAAA1160.12022095782938988No Hit
CTCATCCTCTGGTGCTATCGACCAAAACCCAAGCACTTTCCAAGAAAACC1150.11918457026189515No Hit
CACAGAGGCAGGGTCCCCATGCTGTGTCCCAAGACTGTCTCCTGATAAGA1130.11711179512690566No Hit
CTGCAGGGGGGCTGCAATAGGCTTTGCTCTGAAAGTGCACTTGGGAGAGA1130.11711179512690566No Hit
GGCTGATGGTGGCAGGACCAGTGCTGAGCCCGATGAGCTTCTGGAGGCAG1130.11711179512690566No Hit
TCCCTATACATGATAGCCAGCTCAGCATTTGTACTTACAATAAAACAAGA1120.11607540755941093No Hit
ATATATAGAAGCAGATCCAATATTTTCCAGGTAATAGACATCCTGGAACT1120.11607540755941093No Hit
TTTTTAGCCTTTTAATGCTACAGGATTATTTTTCTCAACTAAGTTCATCC1120.11607540755941093No Hit
GCCCTGCCTGGTTCATGCCATAGAAGAGGGCTTTGCTTCTCAAGTCGCAG1110.11503901999191618No Hit
AGTTTACACACGCCCAGCGTGCCAAAGCCAGTCGGTAAGGGGCAAGCCTC1110.11503901999191618No Hit
GTCTGACAGTGATAAGACAAGGGACAGTGGCTTTAAACTAAATGAGGGGA1100.11400263242442144No Hit
GAGCAGGAGGCGGCCCTGCCTGGTTCATGCCATAGAAGAGGGCTTTGCTT1100.11400263242442144No Hit
CAACAGGATGGAGCTGCCAAGACCAATCCCTGGCCAGAAGCTCCCTTTCC1090.1129662448569267No Hit
CCTTGCCACTGTCAAAAGAAAAAGCACCCACAGAGGCAGGGTCCCCATGC1080.11192985728943196No Hit
GATTAGGATAGATCATAGAATCACAGAATGGCTTAAGTTGGAGGGGACCT1080.11192985728943196No Hit
CTTCTGGAGGCAGGAGGAGCAGGAGGCGGCCCTGCCTGGTTCATGCCATA1080.11192985728943196No Hit
CAAAAGGCACTGATCCCGTGCTACCTGCAGGGGGGCTGCAATAGGCTTTG1070.11089346972193721No Hit
ACCCTGGATCCTTGCCACTGTCAAAAGAAAAAGCACCCACAGAGGCAGGG1070.11089346972193721No Hit
ACTCTGGGGTGGCGAGAGGGAAAGGGAGCTTCTGGCCAGGGATTGGTCTT1060.10985708215444248No Hit
GCCCACCCCACACCGCAGCCCACTGCCGTGCTACAAGGGCAGGAGCGAGC1060.10985708215444248No Hit
CTTTAGATGAAAACACAAAGTCATGTATCATGCTAATTGAAATGATGGGC1050.10882069458694774No Hit
ACCCCACACCGCAGCCCACTGCCGTGCTACAAGGGCAGGAGCGAGCATCC1040.10778430701945299No Hit
GTATTGAGTTTAGTGAGAAAATTCACCCTTGCGATTCTGAAAGTGGTTTT1040.10778430701945299No Hit
ATCCTTGCCACTGTCAAAAGAAAAAGCACCCACAGAGGCAGGGTCCCCAT1040.10778430701945299No Hit
TTAGAACCCTCTGGAAGGAAATATTTAAGTAACACTTTAGATGAAAACAC1030.10674791945195826No Hit
TCCCAAGACTGTCTCCTGATAAGAGCTTTTTGCTGTGGTACCCCGAGGGG1030.10674791945195826No Hit
GCTTATAGAAACTATATTTTTAAGCCAAAACCCATTAAAGGAAAAGCAGA1020.10571153188446351No Hit
CTCTGGGCACTTTGCCCTACCAGTTCCCCTTCCAGCTGCTGCACGAGGCG1020.10571153188446351No Hit
GGTCTTGGCAGCTCCATCCTGTTGGGAGTGGGAACCGCAGGAGCGAGGTG1010.10467514431696877No Hit
GGCAGGACCAGTGCTGAGCCCGATGAGCTTCTGGAGGCAGGAGGAGCAGG1000.10363875674947404No Hit
GATAAGAGCTTTTTGCTGTGGTACCCCGAGGGGCTGCTGAGGGGGGGACC1000.10363875674947404No Hit
GATCCCGTGCTACCTGCAGGGGGGCTGCAATAGGCTTTGCTCTGAAAGTG990.10260236918197929No Hit
GCACCCACCCACCCCCGACTCGTGCAAGCCTGGGGAGGGGCCGCGAAGGT990.10260236918197929No Hit
GTCTCCTGATAAGAGCTTTTTGCTGTGGTACCCCGAGGGGCTGCTGAGGG980.10156598161448455No Hit
GTGTATGGCTTTGTGAGGGAGTGCCTGGAGCGGGTGATTCCTGCCGAGCT970.10052959404698981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)