FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_1971_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_1971_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328152
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGAT8080.2462273580535849No Hit
CTCGTATGCTCAGCAGCAGCAACAGCCTCAGTCTCAAGCTCCACAGCTTC6650.20264999146736876No Hit
GGAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATG6630.2020405178088203No Hit
GAATATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGA5370.16364367732026622No Hit
ATGCAGGCTGCTGCTGCTGCGGCTGTGACTGAGGGTATGAAGATGACTGC4600.14017894146614984No Hit
GCTTCAGACATCGCAACCTGCGTATTCTCAGCAGCAGTCTCAGCCACCCC4540.1383505204905044No Hit
GTGTAAGACCCTACACTTGCTTTTGTTGAACCTCATCCGTTTTCTTACTG4120.12555157366098635No Hit
GTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTGCTGAGTTT4040.12311367902679245No Hit
GACTGAGGGTATGAAGATGACTGCTGAGAAAGAGCCGAGGAAGCTGTCTG3760.11458104780711377No Hit
GTCTCAAGCTCCACAGCTTCAGACATCGCAACCTGCGTATTCTCAGCAGC3630.11061946902654868No Hit
GGATAAAGAAAAGGATACGCAAAAGAAGCGAACGGAAATTTCAGAGATAA3550.10818157439235476No Hit
GTATTACTCCATAGGTAAAGTTCTGGCCAAAAATCTTTGGGACTGTTGCC3370.10269631146541847No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)