FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_1970_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_1970_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9037
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGAATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTT680.7524621002545092No Hit
TTTCTGAACAAACGTTGAAATGGGCATTTCTGGAATGTAGGAGGTGATTT510.5643465751908819No Hit
CTATTAATTGTGACGAGGACTTCCACACCCTCCACGGTGCTGCGGCGCGT490.5422153369481022No Hit
CTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAACTCCACTTCTCC430.4758216222197632No Hit
TGCTAGGGGTGATCAGAGCAGCCACTTCAGCGATGTACATCTTCAGAACA430.4758216222197632No Hit
ACCCAGGGAAGGATGTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAG420.46475600309837334No Hit
ATGTACTTGTGCTGGGAAGGACTTGGGCTGAAGAGGTGGTGGGAAGCCCC410.4536903839769835No Hit
CCCTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGTGTG400.44262476485559366No Hit
CCCCTAGCACTTGCAGAAGGCACCAGAAGGCCATCTCCGTGGGAGATACA390.4315591457342038No Hit
CAAGTACATCATGCAACATCTTTAAAGAAGACTGCTCCTTTCTCCTTTCC380.42049352661281403No Hit
CTTCCACACCCTCCACGGTGCTGCGGCGCGTGCACACATCAGTGTGGGAG370.40942790749142416No Hit
GTTCTGAAGATGTACATCGCTGAAGTGGCTGCTCTGATCACCCCTAGCAC360.3983622883700343No Hit
GGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGTGTGGACTTCAACA360.3983622883700343No Hit
GTGCTGCGGCGCGTGCACACATCAGTGTGGGAGAGGGGCCATGACAGGGT360.3983622883700343No Hit
GTGATGGAAGGATGGTTTGAGGAGTCACTGGGTGAGCTGGAAGTGAACAG340.3762310501272546No Hit
GCCTTGACCTGTATCTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAG340.3762310501272546No Hit
CATCAGTGTGGGAGAGGGGCCATGACAGGGTGGGGGTCATTTCTGGGTGA330.3651654310058648No Hit
CCCCCAGCATCTCATCTTTAAGAGAAAACTCCACTTCTCCCTTCTACAGG320.3540998118844749No Hit
ATGTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAGTGCTGAGACTGT320.3540998118844749No Hit
GTGTGGGAGAGGGGCCATGACAGGGTGGGGGTCATTTCTGGGTGAAATTC310.3430341927630851No Hit
GTCCACACCTGGTGCTGATGCCTTTTCCAGGCTGCTTTTAGCCTGTAGAA300.33196857364169524No Hit
AACCAGAAATGCTTTTTTCTCCCGGCAGTGGCTTGACTTTCACAGCCTTT300.33196857364169524No Hit
GGTCAAGGCGGTTCAATGAGGAAGGACTTTTCTATTTGGGAAAGGAGAAA300.33196857364169524No Hit
GGTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATATGCTGGCAAAGG300.33196857364169524No Hit
GGCCATGACAGGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATT300.33196857364169524No Hit
GGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAACGTTGAA280.3098373353989156No Hit
GTGTTAATTTGTGCTCTATTAATTGTGACGAGGACTTCCACACCCTCCAC280.3098373353989156No Hit
GTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCCGGCA270.29877171627752575No Hit
CCTTTCTCCTTTCCCAAATAGAAAAGTCCTTCCTCATTGAACCGCCTTGA260.2877060971561359No Hit
CTCCACGGTGCTGCGGCGCGTGCACACATCAGTGTGGGAGAGGGGCCATG250.276640478034746No Hit
GGAGAAAGGAGCAGTCTTCTTTAAAGATGTTGCATGATGTACTTGTGCTG250.276640478034746No Hit
GACTTGGGCTGAAGAGGTGGTGGGAAGCCCCCAGAGAAGGGAAGGTCTGT250.276640478034746No Hit
GTGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTG240.2655748589133562No Hit
GTTCAATGAGGAAGGACTTTTCTATTTGGGAAAGGAGAAAGGAGCAGTCT240.2655748589133562No Hit
GTTCTGATTGAGAAGACATGAAAAGAAGTGCTGAGACTGTGGATACAGAT230.2545092397919663No Hit
TGACAGGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGA230.2545092397919663No Hit
GGTTTGAGGAGTCACTGGGTGAGCTGGAAGTGAACAGGGGCATTTGTGCC220.2434436206705765No Hit
CTACTGGACCCTTCTGGGTGCTCGTTCTTGTCTCACATCTCCCTCTCCAC220.2434436206705765No Hit
TTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAACGTTGAAATGGG220.2434436206705765No Hit
GAAGTGAACAGGGGCATTTGTGCCACCCAGGGAAGGATGTTAAGCAGTTC220.2434436206705765No Hit
GCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAATGTAGGAGGTGA220.2434436206705765No Hit
GTCACTGGGTGAGCTGGAAGTGAACAGGGGCATTTGTGCCACCCAGGGAA220.2434436206705765No Hit
CTTTAAAGAAGACTGCTCCTTTCTCCTTTCCCAAATAGAAAAGTCCTTCC210.23237800154918667No Hit
GATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTGGACCCTTCT210.23237800154918667No Hit
TCTTTAAAGAAGACTGCTCCTTTCTCCTTTCCCAAATAGAAAAGTCCTTC210.23237800154918667No Hit
TCCCAGCACAAGTACATCATGCAACATCTTTAAAGAAGACTGCTCCTTTC210.23237800154918667No Hit
TCATTGAACCGCCTTGACCTGTATCTCCCACGGAGATGGCCTTCTGGTGC210.23237800154918667No Hit
GTGCTGGGAAGGACTTGGGCTGAAGAGGTGGTGGGAAGCCCCCAGAGAAG200.22131238242779683No Hit
GAATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCC200.22131238242779683No Hit
CTTCAGCGATGTACATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAG190.21024676330640701No Hit
AGCGAAAGCAGGTGGCAGCAAGCAGGACAGTGATGGAAGGATGGTTTGAG190.21024676330640701No Hit
GCCTTTGCCAGCATATTCCCCTCTGAATGAGCTTTTGTTGAAGTCCACAC190.21024676330640701No Hit
ATGCTACACCGTGGCTGATCCTCAAAACCCTCATTTATCTTAAGTAAAAG190.21024676330640701No Hit
ACAGGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAAC190.21024676330640701No Hit
GTGCTAGGGGTGATCAGAGCAGCCACTTCAGCGATGTACATCTTCAGAAC180.19918114418501714No Hit
GCTCTGATCACCCCTAGCACTTGCAGAAGGCACCAGAAGGCCATCTCCGT180.19918114418501714No Hit
GGGAGATACAGGTCAAGGCGGTTCAATGAGGAAGGACTTTTCTATTTGGG180.19918114418501714No Hit
GCATATTCCCCTCTGAATGAGCTTTTGTTGAAGTCCACACCTGGTGCTGA180.19918114418501714No Hit
GGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAA180.19918114418501714No Hit
AAAGAAGACTGCTCCTTTCTCCTTTCCCAAATAGAAAAGTCCTTCCTCAT180.19918114418501714No Hit
CTTCTGGGTGCTCGTTCTTGTCTCACATCTCCCTCTCCACCTCCACAAAC180.19918114418501714No Hit
CCAGAAGGCCATCTCCGTGGGAGATACAGGTCAAGGCGGTTCAATGAGGA180.19918114418501714No Hit
TCTTTAAAGATGTTGCATGATGTACTTGTGCTGGGAAGGACTTGGGCTGA180.19918114418501714No Hit
GCACCAGGTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATATGCTGG170.1881155250636273No Hit
TCCACACCCTCCACGGTGCTGCGGCGCGTGCACACATCAGTGTGGGAGAG170.1881155250636273No Hit
GGAATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCC170.1881155250636273No Hit
ATACAGGTCAAGGCGGTTCAATGAGGAAGGACTTTTCTATTTGGGAAAGG170.1881155250636273No Hit
GGACAGTGATGGAAGGATGGTTTGAGGAGTCACTGGGTGAGCTGGAAGTG170.1881155250636273No Hit
GATCAGAGCAGCCACTTCAGCGATGTACATCTTCAGAACACCCCCAGCAT170.1881155250636273No Hit
AAGCAGGACAGTGATGGAAGGATGGTTTGAGGAGTCACTGGGTGAGCTGG160.17704990594223746No Hit
GCCCAAGTCCTTCCCAGCACAAGTACATCATGCAACATCTTTAAAGAAGA160.17704990594223746No Hit
TGCTGGGGGTGTTCTGAAGATGTACATCGCTGAAGTGGCTGCTCTGATCA160.17704990594223746No Hit
GCACACATCAGTGTGGGAGAGGGGCCATGACAGGGTGGGGGTCATTTCTG150.16598428682084762No Hit
CTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGTGTGGA150.16598428682084762No Hit
TCCCACCACCTCTTCAGCCCAAGTCCTTCCCAGCACAAGTACATCATGCA150.16598428682084762No Hit
GATGTACATCGCTGAAGTGGCTGCTCTGATCACCCCTAGCACTTGCAGAA150.16598428682084762No Hit
GTTCACAGACATTCGGTTTGTTTCTCCCTTTTGGTGATTGTGAGTAATGC150.16598428682084762No Hit
TCCCTTCTCTGGGGGCTTCCCACCACCTCTTCAGCCCAAGTCCTTCCCAG150.16598428682084762No Hit
GTCCTTCCCAGCACAAGTACATCATGCAACATCTTTAAAGAAGACTGCTC150.16598428682084762No Hit
TCGCTGAAGTGGCTGCTCTGATCACCCCTAGCACTTGCAGAAGGCACCAG140.1549186676994578No Hit
GAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTAGGGGTGATCAGAGCAGC140.1549186676994578No Hit
TCTCCCTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGCACCAGGT140.1549186676994578No Hit
GGCTGTGAAAGTCAAGCCACTGCCGGGAGAAAAAAGCATTTCTGGTTCGT140.1549186676994578No Hit
TTCACAGCCTTTGCCAGCATATTCCCCTCTGAATGAGCTTTTGTTGAAGT140.1549186676994578No Hit
GAACAGGGGCATTTGTGCCACCCAGGGAAGGATGTTAAGCAGTTCTGATT140.1549186676994578No Hit
GAGAAGGGAAGGTCTGTGGATCCTGGTGAGGGTTTGTGGAGGTGGAGAGG140.1549186676994578No Hit
GAAAAGAAGTGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGAGTG140.1549186676994578No Hit
GCTTTTAGCCTGTAGAAGGGAGAAGTGGAGTTTTCTCTTAAAGATGAGAT140.1549186676994578No Hit
GTACATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAACTCCAC140.1549186676994578No Hit
CTGCAAGTGCTAGGGGTGATCAGAGCAGCCACTTCAGCGATGTACATCTT140.1549186676994578No Hit
GTATCTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTAGGGGT140.1549186676994578No Hit
GTTCTATGTATGTGGAATGTCAGAATAGGCAAATCTAGAGAGGCACTAAG140.1549186676994578No Hit
GACCTGTATCTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTA140.1549186676994578No Hit
AATTCAGGCCCAGCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAA130.14385304857806794No Hit
CCACAAACCCTCACCAGGATCCACAGACCTTCCCTTCTCTGGGGGCTTCC130.14385304857806794No Hit
CTCCACTTCTCCCTTCTACAGGCTAAAAGCAGCCTGGAAAAGGCATCAGC130.14385304857806794No Hit
AGCTGGAAGTGAACAGGGGCATTTGTGCCACCCAGGGAAGGATGTTAAGC130.14385304857806794No Hit
ATGTACATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAACTCC130.14385304857806794No Hit
TCCACAGACCTTCCCTTCTCTGGGGGCTTCCCACCACCTCTTCAGCCCAA120.1327874294566781No Hit
AGCGAAAGCAGGGAAAAGACAGGAAACAGGTGTTGTGTTAATTTGTGCTC120.1327874294566781No Hit
GATGAGATGCTGGGGGTGTTCTGAAGATGTACATCGCTGAAGTGGCTGCT120.1327874294566781No Hit
GGGAAAAGACAGGAAACAGGTGTTGTGTTAATTTGTGCTCTATTAATTGT120.1327874294566781No Hit
GGATAAAATTACTCAATAGAAAAATAACAAATAGTGGCAAGGATGAGTAG120.1327874294566781No Hit
CAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTGGACCCTTCTGGGTGCTC120.1327874294566781No Hit
GGAATATGCTGGCAAAGGCTGTGAAAGTCAAGCCACTGCCGGGAGAAAAA120.1327874294566781No Hit
CGTTGAAATGGGCATTTCTGGAATGTAGGAGGTGATTTTTTGAAACGAAC120.1327874294566781No Hit
AAGCAGTTCTGATTGAGAAGACATGAAAAGAAGTGCTGAGACTGTGGATA120.1327874294566781No Hit
GTGTGGACTTCAACAAAAGCTCATTCAGAGGGGAATATGCTGGCAAAGGC120.1327874294566781No Hit
TGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCCGGC120.1327874294566781No Hit
GACTTTCACAGCCTTTGCCAGCATATTCCCCTCTGAATGAGCTTTTGTTG120.1327874294566781No Hit
GTATTCCTGTGTCCTAAGGCCAATTACTTGAGTTGGAATTCTCCATTTAG120.1327874294566781No Hit
GGCCCAGCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAATGTAGG120.1327874294566781No Hit
ACTGGACCCTTCTGGGTGCTCGTTCTTGTCTCACATCTCCCTCTCCACCT120.1327874294566781No Hit
GAATATAGCAAAGGATTTAAAGAGACATTTCTCCAAAGATATACAAATGG120.1327874294566781No Hit
GATCACCCCTAGCACTTGCAGAAGGCACCAGAAGGCCATCTCCGTGGGAG120.1327874294566781No Hit
GGCTTGACTTTCACAGCCTTTGCCAGCATATTCCCCTCTGAATGAGCTTT120.1327874294566781No Hit
CTCTGGGGGCTTCCCACCACCTCTTCAGCCCAAGTCCTTCCCAGCACAAG110.12172181033528826No Hit
GTTATGAGTTTTTAAGATATATTCTAGATACGAGTCCCTTTTAGATAACC110.12172181033528826No Hit
CTTCAGCCCAAGTCCTTCCCAGCACAAGTACATCATGCAACATCTTTAAA110.12172181033528826No Hit
ATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTGGACC110.12172181033528826No Hit
GGAGAGGGAGATGTGAGACAAGAACGAGCACCCAGAAGGGTCCAGTAGCC110.12172181033528826No Hit
GCGATGTACATCTTCAGAACACCCCCAGCATCTCATCTTTAAGAGAAAAC110.12172181033528826No Hit
GGGAAGGATGTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAGTGCTG110.12172181033528826No Hit
TTATTTATCCATTTATCAGTTCACAGACATTCGGTTTGTTTCTCCCTTTT110.12172181033528826No Hit
GTGGTGGGAAGCCCCCAGAGAAGGGAAGGTCTGTGGATCCTGGTGAGGGT110.12172181033528826No Hit
TGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGG110.12172181033528826No Hit
ATATTCCCCTCTGAATGAGCTTTTGTTGAAGTCCACACCTGGTGCTGATG110.12172181033528826No Hit
TCTCTGGGGGCTTCCCACCACCTCTTCAGCCCAAGTCCTTCCCAGCACAA110.12172181033528826No Hit
GCCTGGAAAAGGCATCAGCACCAGGTGTGGACTTCAACAAAAGCTCATTC110.12172181033528826No Hit
GTGGATACAGATAAGCTCAGTTGGTTGAGTGAAGGTTTGGGGGGCTACTG110.12172181033528826No Hit
ATTCAGGCCCAGCATTTCTGAACAAACGTTGAAATGGGCATTTCTGGAAT110.12172181033528826No Hit
GAAGATGTACATCGCTGAAGTGGCTGCTCTGATCACCCCTAGCACTTGCA110.12172181033528826No Hit
TTCTGCAAGTGCTAGGGGTGATCAGAGCAGCCACTTCAGCGATGTACATC110.12172181033528826No Hit
GTGAAATTCAGGCCCAGCATTTCTGAACAAACGTTGAAATGGGCATTTCT110.12172181033528826No Hit
ATGACAGGGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTG110.12172181033528826No Hit
GAAGAGGTGGTGGGAAGCCCCCAGAGAAGGGAAGGTCTGTGGATCCTGGT110.12172181033528826No Hit
CTTAAAGATGAGATGCTGGGGGTGTTCTGAAGATGTACATCGCTGAAGTG100.11065619121389841No Hit
ATAGAACACAGTTCAATTGGGCTTCACCAAAATTAAAAACTTTGGTGCTT100.11065619121389841No Hit
CCTGGTGCTGATGCCTTTTCCAGGCTGCTTTTAGCCTGTAGAAGGGAGAA100.11065619121389841No Hit
TTCCAGAAATGCCCATTTCAACGTTTGTTCAGAAATGCTGGGCCTGAATT100.11065619121389841No Hit
GGGAAGGACTTGGGCTGAAGAGGTGGTGGGAAGCCCCCAGAGAAGGGAAG100.11065619121389841No Hit
CACCTGGTGCTGATGCCTTTTCCAGGCTGCTTTTAGCCTGTAGAAGGGAG100.11065619121389841No Hit
CATCTCATCTTTAAGAGAAAACTCCACTTCTCCCTTCTACAGGCTAAAAG100.11065619121389841No Hit
GTTAAGCAGTTCTGATTGAGAAGACATGAAAAGAAGTGCTGAGACTGTGG100.11065619121389841No Hit
GCACAAGTACATCATGCAACATCTTTAAAGAAGACTGCTCCTTTCTCCTT100.11065619121389841No Hit
TCTTTAAGAGAAAACTCCACTTCTCCCTTCTACAGGCTAAAAGCAGCCTG100.11065619121389841No Hit
CTCCCACGGAGATGGCCTTCTGGTGCCTTCTGCAAGTGCTAGGGGTGATC100.11065619121389841No Hit
GAAATGCTTTTTTCTCCCGGCAGTGGCTTGACTTTCACAGCCTTTGCCAG100.11065619121389841No Hit
GAAATGGGCATTTCTGGAATGTAGGAGGTGATTTTTTGAAACGAACCAGA100.11065619121389841No Hit
GGTGGGGGTCATTTCTGGGTGAAATTCAGGCCCAGCATTTCTGAACAAAC100.11065619121389841No Hit
GTATGTAACCAAACAGCCAAGTGCATATCTTTATTGCACAGATTTTCTTT100.11065619121389841No Hit
ATGTTGCATGATGTACTTGTGCTGGGAAGGACTTGGGCTGAAGAGGTGGT100.11065619121389841No Hit
GGGGAATATGCTGGCAAAGGCTGTGAAAGTCAAGCCACTGCCGGGAGAAA100.11065619121389841No Hit
ATGTAGGAGGTGATTTTTTGAAACGAACCAGAAATGCTTTTTTCTCCCGG100.11065619121389841No Hit
CTTTAAAGATGTTGCATGATGTACTTGTGCTGGGAAGGACTTGGGCTGAA100.11065619121389841No Hit
CATTTGTGCCACCCAGGGAAGGATGTTAAGCAGTTCTGATTGAGAAGACA100.11065619121389841No Hit
GAGGGGAATATGCTGGCAAAGGCTGTGAAAGTCAAGCCACTGCCGGGAGA100.11065619121389841No Hit
CTATTGTTTTGCCCATAAAAAGAAACGAAATACTGATACATGCTACACCG100.11065619121389841No Hit
GTCTGACAGTTTCTCAAAAAGTAAAACATAAAATTGCCATGTGACCAGCA100.11065619121389841No Hit
CTCCTTTCCCAAATAGAAAAGTCCTTCCTCATTGAACCGCCTTGACCTGT100.11065619121389841No Hit
CATGAAAAGAAGTGCTGAGACTGTGGATACAGATAAGCTCAGTTGGTTGA100.11065619121389841No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)