FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_1970_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_1970_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145029
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAAGGTCCTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCG17931.236304463245282No Hit
GGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTA12240.8439691372070414No Hit
AGCGAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTG11770.8115618255659213No Hit
AAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAG11460.7901867902281612No Hit
TCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTGCG10670.7357149259803212No Hit
GTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACACA10350.7136503733736012No Hit
GACTGGAGCCGTGCGAGGGGGGCGGCGCAGCCTGCGGCCTTCGGCGAGAC9230.6364244392500811No Hit
CGTCCACACAGGGTAAGGTCCTTTAGTTCAAGGTGCACATCCCGAATGCC8490.5854001613470409No Hit
GCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGC8130.560577539664481No Hit
CAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCG7990.5509242978990408No Hit
GGATATCGGTGGGACCTTGGTCAAACTTGTCTATTTTGAACCAAAGGACA7330.5054161581476808No Hit
CATTACAGCTTCTCAAAAGCTCTTTAAAAACCACCGGAAGAACCCAATGT6640.45783946658944075No Hit
GCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTA6580.4537023629756807No Hit
GGCATGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCC6100.4206055340656007No Hit
GCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGG5950.4102627750312006No Hit
GTGCGAGGGGGGCGGCGCAGCCTGCGGCCTTCGGCGAGACTCGGTCCGCA5600.3861296706176006No Hit
GCCTGCGGCCTTCGGCGAGACTCGGTCCGCAAGAACCGCCCGCTCTTCCC5570.38406111881072064No Hit
TCCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTT5210.3592384971281606No Hit
GTGGTATAACATGAATTTTCAAAAAGCAGGGGATTCCTGTAATTCCAAAT5050.3482062208248005No Hit
GTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAG4970.3426900826731205No Hit
ATCTGGAACTTTCCCCTCCTTCTTTCTTCAGACCTCCTGGCCTGGGGAGG4940.34062153086624053No Hit
TCCCTTGGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTAT4700.3240731164112005No Hit
CGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCG4670.3220045646043205No Hit
GGACTGGAGCCGTGCGAGGGGGGCGGCGCAGCCTGCGGCCTTCGGCGAGA4660.32131504733536054No Hit
GAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGT4440.3061456674182405No Hit
AAAGCAGGCATGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTT4420.30476663288032046No Hit
GCCTTTACGTCCACACAGGGTAAGGTCCTTTAGTTCAAGGTGCACATCCC4150.28614966661840047No Hit
AGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCG4150.28614966661840047No Hit
TGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGCCTTTACGTCCACAC3980.27442787304608046No Hit
GTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTTTCAAAAAG3690.2544318722462404No Hit
CGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTAG3630.2502947686324804No Hit
GTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCAGGT3620.2496052513635204No Hit
GGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGC3430.2365044232532804No Hit
GTATAACATGAATTTTCAAAAAGCAGGGGATTCCTGTAATTCCAAATAGC3390.23374635417744036No Hit
CAGTAGGACAGGCCAGCGGCAGGAGAGGATTCAGGCCCCGTGAGGGAAGG3370.23236731963952034No Hit
GTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCGTCCTCCTC3310.22823021602576035No Hit
GTCCTGAGATGGAGCCCCGCATTGGGCTCTCTGCTCAGCGGGGAGCCTAC3280.22616166421888037No Hit
ACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCG3190.21995600879824037No Hit
AGCAAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTG3070.21168180157072033No Hit
AGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGA3070.21168180157072033No Hit
AGCGTATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTT3060.21099228430176034No Hit
ACTTTATATCCTTTAGATTTGCCAACTAGCATATGTGTCCATTACATACT2970.20478662888112034No Hit
GCGAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGG2940.20271807707424033No Hit
TCTCAGGACCCTGAGATCATAACCTGAGCCGAAGGCAGAGGCTCTAACCC2900.19996000799840033No Hit
GGTTATGATCTCAGGGTCCTGAGATGGAGCCCCGCATTGGGCTCTCTGCT2850.1965124216536003No Hit
GGTCAAACTTGTCTATTTTGAACCAAAGGACATCACTGCCGAAGAGGAGG2830.19513338711568032No Hit
GTCCGCAAGAACCGCCCGCTCTTCCCTTGGTTTGGCCTGGATATCGGTGG2800.1930648353088003No Hit
GCCCCAGGCTGCGCACAGAAAGGCAGGGGTTCTGGGGCACCTGGGTGGCT2780.1916858007708803No Hit
GGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGCGGCGC2760.1903067662329603No Hit
GCAAAGAGGTAGGCAGAGAGAGAGAGAGAAAGGGAAGTAGGCTCCCCGCT2760.1903067662329603No Hit
CTCTTCCCTTGGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGT2740.1889277316950403No Hit
GTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTAGTTCAAGGT2710.1868591798881603No Hit
GTCCTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCA2670.18410111081232028No Hit
TTCTACTTCTTCGTCCTCCTCTTCGGCAGTGATGTCCTTTGGTTCAAAAT2670.18410111081232028No Hit
CCTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACG2660.18341159354336029No Hit
GGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGC2650.1827220762744003No Hit
ATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGC2570.17720593812272029No Hit
GTGTATGTCCAAAGAAGATCCAGTTCATCATAGGCATTTGCTCCCTTCAG2530.17444786904688028No Hit
GTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAGGTCCCACCGAT2480.17100028270208026No Hit
TTTTTGAGGTTTTCTACTTCTTCGTCCTCCTCTTCGGCAGTGATGTCCTT2440.16824221362624026No Hit
GGATATACCATTTGGCCTAGTAATTTTACTTCTGCAAACCGATTCAATAG2430.16755269635728026No Hit
GCCTTGACCTATCTGGAACTTTCCCCTCCTTCTTTCTTCAGACCTCCTGG2430.16755269635728026No Hit
AGCGAAAGCAGGCATGTCATGGGTAGGAAAGCGTATAAAGTGCAGATTGC2410.16617366181936027No Hit
GGTATAACATGAATTTTCAAAAAGCAGGGGATTCCTGTAATTCCAAATAG2380.16410511001248027No Hit
GAGGTAGGCAGAGAGAGAGAGAGAAAGGGAAGTAGGCTCCCCGCTGAGCA2360.16272607547456025No Hit
TCTTCGTCCTCCTCTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAG2340.16134704093664026No Hit
CATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTG2330.16065752366768027No Hit
CATAGAGCCTGGACTAGATTTTATTTGCTTTTGAGTGCCTTTTTGAAAGC2320.15996800639872025No Hit
ATTTTGAACCAAAGGACATCACTGCCGAAGAGGAGGACGAAGAAGTAGAA2310.15927848912976025No Hit
CTCTATATGTGAATAGAATCTCTTTACAGGAACAGACAGAAAGGCAACAG2310.15927848912976025No Hit
TGTCTATTTTGAACCAAAGGACATCACTGCCGAAGAGGAGGACGAAGAAG2290.15789945459184024No Hit
GCTGTAATGTGATCAGCTTCTGGGCTTATTCCAGACGATGAGGCTGCTCA2270.15652042005392025No Hit
AGTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTTTCAAAAA2260.15583090278496026No Hit
GGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTATTTTGAACCAAA2260.15583090278496026No Hit
GGCCAGCGGCAGGAGAGGATTCAGGCCCCGTGAGGGAAGGAAATCACCAG2240.15445186824704024No Hit
GCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAG2240.15445186824704024No Hit
GTACGAGTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTTTC2220.15307283370912025No Hit
GGCCTGGGGAGGATGAAGATGCATGTCCCAGGCAAGCAACCTGGAGACAA2220.15307283370912025No Hit
GACTGGCGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGCG2190.15100428190224024No Hit
GACATACACAGCTCAAAAAAGAAAGTCCCACTGAAGCAGCTTTCAAAAAG2190.15100428190224024No Hit
CTTGAATACATGGTAAACTTTTTTGCTCTCATAGAGCCTGGACTAGATTT2180.15031476463328025No Hit
CCTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTT2120.14617766101952023No Hit
CTCTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAGG2120.14617766101952023No Hit
ACCCAATGTAATCAGCAAAGATGGAGCAGCAGGAGGGGGAAGAGGAAGAG2110.14548814375056024No Hit
CTCAAGGCCTTGACCTATCTGGAACTTTCCCCTCCTTCTTTCTTCAGACC2080.14341959194368023No Hit
GCCGAAGAGGAGGACGAAGAAGTAGAAAACCTCAAAAACATCCGCAAGTA2070.14273007467472024No Hit
GTTCTGGGGCACCTGGGTGGCTCAGAGGGTTAGAGCCTCTGCCTTCGGCT2070.14273007467472024No Hit
GTGGTAGAGATGATAAAAGGAAACATGAATAAATGTTTCCGATAAAAAAA2070.14273007467472024No Hit
CGCTGGAAGCGCGGCGGCGGGACTGGAGCCGTGCGAGGGGGGCGGCGCAG2060.14204055740576021No Hit
AAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGTTTGATGTCA2040.14066152286784023No Hit
ATAAAGTGCAGATTGCCTTTACGTCCACACAGGGTAAGGTCCTTTAGTTC2030.13997200559888023No Hit
GGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTATTTTGAA2020.1392824883299202No Hit
TCCCAGGCAAGCAACCTGGAGACAAAGCGATGAGCAGCCTCATCGTCTGG2000.13790345379200022No Hit
GTCCAGCGCGCGGTCAATGTCGGGGATGCGGTCAAGGCGGGACAGCTGAT1990.13721393652304023No Hit
CCCTTGGTTTGGCCTGGATATCGGTGGGACCTTGGTCAAACTTGTCTATT1990.13721393652304023No Hit
GCAAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGG1990.13721393652304023No Hit
GAGCAAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCT1970.13583490198512022No Hit
GAAGAACCCAATGTAATCAGCAAAGATGGAGCAGCAGGAGGGGGAAGAGG1970.13583490198512022No Hit
GTCTGGAATAAGCCCAGAAGCTGATCACATTACAGCTTCTCAAAAGCTCT1970.13583490198512022No Hit
CGGTGGGACCTTGGTCAAACTTGTCTATTTTGAACCAAAGGACATCACTG1970.13583490198512022No Hit
GGTACGAGTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTTT1950.13445586744720023No Hit
CTGTGGATCCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTG1940.1337663501782402No Hit
GAAGAAAGGCAGGGCTTCTAAGGGCCCGGTCTCTGGAGCCTTCTGCTGCC1930.1330768329092802No Hit
CTCATAGAGCCTGGACTAGATTTTATTTGCTTTTGAGTGCCTTTTTGAAA1930.1330768329092802No Hit
GGGCGGCGCAGCCTGCGGCCTTCGGCGAGACTCGGTCCGCAAGAACCGCC1900.1310082811024002No Hit
CGCAAGAACCGCCCGCTCTTCCCTTGGTTTGGCCTGGATATCGGTGGGAC1870.1289397292955202No Hit
GAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGG1850.1275606947576002No Hit
GTCAAAGACTGACCTTATTAAGAAAGCAGTGACCTCTTGAATACATGGTA1850.1275606947576002No Hit
CTTTAAAAACCACCGGAAGAACCCAATGTAATCAGCAAAGATGGAGCAGC1820.1254921429507202No Hit
CTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAGGTC1820.1254921429507202No Hit
TTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCGTC1790.12342359114384019No Hit
TCACATTACAGCTTCTCAAAAGCTCTTTAAAAACCACCGGAAGAACCCAA1790.12342359114384019No Hit
CCTCTTCGGCAGTGATGTCCTTTGGTTCAAAATAGACAAGTTTGACCAAG1790.12342359114384019No Hit
GTACTTGCGGATGTTTTTGAGGTTTTCTACTTCTTCGTCCTCCTCTTCGG1790.12342359114384019No Hit
GGATGGGACTGAGAAACAGATCTACTTTATATCCTTTAGATTTGCCAACT1790.12342359114384019No Hit
AAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGC1790.12342359114384019No Hit
GCTTTGGTGGGTGGCAGCTGGGAGCCTTTATGGCCCCAGGCTGCGCACAG1780.12273407387488018No Hit
TTTATATACATGCATTTTTAAGTAAACTCTACTCCCAACATGGGGCTTGA1780.12273407387488018No Hit
CTGTAATTCCAAATAGCCAGTAGGACAGGCCAGCGGCAGGAGAGGATTCA1760.1213550393369602No Hit
GGACATCACTGCCGAAGAGGAGGACGAAGAAGTAGAAAACCTCAAAAACA1730.1192864875300802No Hit
GTCCATTACATACTCAAGGCCTTGACCTATCTGGAACTTTCCCCTCCTTC1730.1192864875300802No Hit
ATATATACAGTATGCATAAGCATTTATATACATGCATTTTTAAGTAAACT1730.1192864875300802No Hit
TCTTTCTTCAGACCTCCTGGCCTGGGGAGGATGAAGATGCATGTCCCAGG1710.11790745299216018No Hit
CCTTTAGATTTGCCAACTAGCATATGTGTCCATTACATACTCAAGGCCTT1700.1172179357232002No Hit
CATATATAGTACACATGTATTTAGTGAACTGTCCTCTTTACATGATGGTA1700.1172179357232002No Hit
ACACAGGGTAAGGTCCTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGG1690.1165284184542402No Hit
ATATAGTACACATGTATTTAGTGAACTGTCCTCTTTACATGATGGTAGCG1680.11583890118528019No Hit
GGATGAAGATGCATGTCCCAGGCAAGCAACCTGGAGACAAAGCGATGAGC1680.11583890118528019No Hit
GGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTTCTACTT1680.11583890118528019No Hit
GGACAGGCCAGCGGCAGGAGAGGATTCAGGCCCCGTGAGGGAAGGAAATC1680.11583890118528019No Hit
GGCTATAGGAAATATTTTGAGCAGCAGAGGTGGGTTGGATTTGGAAAGGG1670.11514938391632018No Hit
ACATTGGGTTCTTCCGGTGGTTTTTAAAGAGCTTTTGAGAAGCTGTAATG1670.11514938391632018No Hit
CGGCGATGGAGCGGCGGCGGGACTGGCGCTGGAAGCGCGGCGGCGGGACT1650.11377034937840019No Hit
CCGATATCCAGGCCAAACCAAGGGAAGAGCGGGCGGTTCTTGCGGACCGA1620.11170179757152018No Hit
CTTTAGTTCAAGGTGCACATCCCGAATGCCTGTGGATCCGTAGGCCACGT1580.10894372849568018No Hit
AGGTACGAGTGTAGGTCTTTTGTGAAAGCAAAGTGGTATAACATGAATTT1560.10756469395776017No Hit
GAATAAGCCCAGAAGCTGATCACATTACAGCTTCTCAAAAGCTCTTTAAA1550.10687517668880016No Hit
CTTTACAGGAACAGACAGAAAGGCAACAGTTGTTTCTGGGGAGGGGATGG1540.10618565941984018No Hit
TACTAAAAGGATATACCATTTGGCCTAGTAATTTTACTTCTGCAAACCGA1540.10618565941984018No Hit
CCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTTTTC1530.10549614215088017No Hit
CTCCTGGCCTGGGGAGGATGAAGATGCATGTCCCAGGCAAGCAACCTGGA1520.10480662488192016No Hit
GCCTTCGGCGAGACTCGGTCCGCAAGAACCGCCCGCTCTTCCCTTGGTTT1520.10480662488192016No Hit
ACCTTGGTCAAACTTGTCTATTTTGAACCAAAGGACATCACTGCCGAAGA1520.10480662488192016No Hit
CGAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCTGGA1510.10411710761296016No Hit
GTACTATACATTTCATTCCCTTCCATACTGGTTTTCATTGATCTTAGGAA1510.10411710761296016No Hit
GTACACATGTATTTAGTGAACTGTCCTCTTTACATGATGGTAGCGTACTA1500.10342759034400018No Hit
GATCCGTAGGCCACGTTTGATGTCAGGTACTTGCGGATGTTTTTGAGGTT1490.10273807307504017No Hit
GTTTTCTACTTCTTCGTCCTCCTCTTCGGCAGTGATGTCCTTTGGTTCAA1490.10273807307504017No Hit
TCCACACAGGGTAAGGTCCTTTAGTTCAAGGTGCACATCCCGAATGCCTG1490.10273807307504017No Hit
GAGCGAAAGCAGGGAACCCGCGGCGATGGAGCGGCGGCGGGACTGGCGCT1480.10204855580608016No Hit
CTTCAGACCTCCTGGCCTGGGGAGGATGAAGATGCATGTCCCAGGCAAGC1480.10204855580608016No Hit
ATCCAGAGTCACAGCTCTACTGACTAAGCCAGCTGGGTGCCTCTATATGT1470.10135903853712017No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)