FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq_1414_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq_1414_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905412
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG31530.16547602303333872No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA25760.13519385833614986No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT25250.13251727185511586No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG24850.13041798834057935No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC21850.1146733619815557No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT20400.10706345924136093No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC20170.10585637122050243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)