FastQCFastQC Report
Wed 12 May 2021
HGGF2BGXJ_n01_ReSeq2_1789_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGGF2BGXJ_n01_ReSeq2_1789_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164478
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC22890.19656876299938686No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG17270.1483067949759463No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15770.13542548678463656No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA14580.1252063156195308No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA13880.11919503846358627No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT12790.10983462117790117No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA11870.1019340854872312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)