FastQCFastQC Report
Sat 2 Sep 2017
HGG5NBGX3_n02_cc11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG5NBGX3_n02_cc11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86888617
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA4128170.4751105659789705No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC3469420.3992951113492807Illumina Single End PCR Primer 1 (96% over 33bp)
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT3233840.3721822387850873No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2679050.30833152747729886No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA2533850.29162047774336197No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT2106440.24242991461125457No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG2015470.23196018875521981No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1919390.22090235364201966No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1829550.21056267934383166No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1792390.20628593961853486No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1585780.1825072207099349No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1548660.1782350845796061No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1471480.16935244808879854No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT1440470.16578351108983586No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA1355720.15602964425132926No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1350810.15546455296900397No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC1273260.1465393332247422No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1258610.14485326656770242No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT1211870.13947396584756322No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG1162870.1338345620117305No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1153940.13280680943511852No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA1146120.1319068066188693No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC1125270.12950718274178538No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1106250.12731817333448867No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA1105800.12726638289109837No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1082350.12456752534109271No Hit
TTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGT1077280.12398401967889533No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1060510.12205396248855015No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG1060510.12205396248855015No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG1054340.12134385796473202No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1050700.1209249308226416No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA1043540.12010088732336481No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT1038690.11954270143349156No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1032120.1187865609599932No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1030900.11864615131346835No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT1022040.11762645502805046No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1016150.11694857566900851No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC1007700.11597606623201288No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA981070.1129112228820491No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT977650.11251761551228281No Hit
GCTGATTCCTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCAT967480.11134715149166202No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG953060.10968755550568839No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC926180.10659393968717444No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA925060.1064650390280697No Hit
GCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGT923950.10633728926770696No Hit
TATTGCTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTG909610.10468690047166938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAG838850.089.315985
GGAAAGG653500.083.817246
GTCCAAT194400.074.840191
GGGTCTG202750.067.6000752
TCCAATC216950.066.698132
GGTCTGG217400.063.8564263
GGGGAGA1399650.062.3133964
CACCTGG865000.058.590943
ACCTGGC872150.057.9899024
GCGAGCG427200.057.0142171
GGGCACT39450.056.709431
CTTGTTA315300.056.3742831
CCGGCAT124600.056.3553851
TGGGTCT168950.056.21321
TCAAAGG292850.056.0009887
GTTACGA322250.054.907684
CCTGGCC930550.054.5170825
TTACGAC342800.051.7522855
GGCATCG124600.051.6316343
CAAAGGA302300.051.0351568
Produced by FastQC (version 0.11.2)