FastQCFastQC Report
Sat 2 Sep 2017
HGG5NBGX3_n02_bc11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG5NBGX3_n02_bc11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10784586
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT551490.5113687256979544No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG437400.40557885114922354No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA409890.38007022244525657No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG268570.2490313490012505No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT264700.24544289414540343No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG251050.23278594097167937No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA247020.22904912622515133No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC239320.22190930648612753No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG225580.2091689008738954No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC214260.1986724386082136No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT213740.1981902689635003No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA212340.1968921199200414No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT209950.19467599405299377No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA208040.19290494785798917No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT205390.19044773716858487No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT202960.18819452132886696No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG199100.18461533896618748No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT191970.17800405133771477No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC177300.16440130386089924No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT175980.1631773347627809No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC173760.16111884127958181No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA164870.15287559485361793No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC157000.1455781427307455No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG155760.14442835357796766No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA143690.13323645432471865No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT143430.132995369502362No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG142950.13255028983031894No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT142630.1322535700489569No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC138920.12881347508379087No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT138510.1284333028639208No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT138450.1283776679049154No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC137280.1272927862043105No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA133130.12344470153977168No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT132580.12293471441555569No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG131420.12185910520811834No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT131130.12159020290625898No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT130840.12132130060439966No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC130600.12109876076837812No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA130170.1207000435621729No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG129610.12018078394478934No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG128300.11896608733983854No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC125100.11599888952621826No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC124600.11553526486784008No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT121500.11266079198589542No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG119250.11057448102319366No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC117670.10910942710271863No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT113910.1056229696717148No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA113370.10512225504066637No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA113270.10502953010899074No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG112120.10396319339472096No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT111740.10361083865435354No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA109920.101923244897857No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA109820.10183051996618137No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA109710.10172852254133817No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA108970.10104235804693847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA47300.073.078291
GTCCAAT25700.072.4436951
ACCTGGC119600.072.37764
CACCTGG125550.069.546933
CCTGGCC127650.068.586865
GGGCACT5200.067.999661
GGCCGAC9800.067.0084151
GTTACGA51350.066.0558554
TCCAATC29000.064.64072
CCGGCAT13100.063.3491171
TTACGAC54800.062.2767525
CTGGCCC147450.059.275596
TGGCCCG147350.059.0693247
GCCCGGA155150.056.0989049
GAGTCTA30200.053.716322
CGCCCTT8550.053.1726651
GGAGTCT30850.052.6309661
GGCGGTT53950.052.5939334
CTCACCT177250.050.524211
ACGACTT68800.050.1225247
Produced by FastQC (version 0.11.2)