FastQCFastQC Report
Sat 2 Sep 2017
HGG5NBGX3_n02_at12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG5NBGX3_n02_at12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6436685
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT184510.2866537666516227No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA140340.21803148670472455No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA128500.1996369249077747No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC123640.19208645444044567No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA116270.18063646115974294No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG110310.17137703647141347No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC108450.1684873502431764No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC100160.15560804979581883No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT95700.14867901722703533No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT91160.14162569707854275No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG90670.1408644356528244No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA88500.1374931350532145No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC88280.13715134420901443No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC86760.13478988019454113No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT85510.13284788676158613No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG77060.11972001115481028No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA73400.11403385438311803No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT73140.11362991974906338No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA72920.1132881289048633No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG71440.11098880868024458No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG69940.10865841656069856No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT69620.10816126624186209No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT68790.10687178260237995No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA65190.10127884151546952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT13850.078.161861
CTTGTTA23900.067.338021
GTTACGA25950.061.053854
GGGCACT3450.058.5723081
TTACGAC27100.058.4525575
CCGGCAT6100.057.972181
AGTCTAA13900.055.4594543
GAGTCTA19650.055.0444642
ATCAAAG22350.053.4753536
CAAAGGA22150.051.0288
CCTGGCC56600.050.631035
GGAGTCT21800.049.657881
TGCCGCA7250.046.785711
TGGCCCG63700.044.528997
GGTCTGG9550.044.5097583
CCAATCA25650.043.8171233
TCAAAGG29250.043.8106967
TCTAACG17450.043.7381675
TTGTGTC4000.041.4622422
GCTTGTA20300.041.1384547
Produced by FastQC (version 0.11.2)