FastQCFastQC Report
Sat 2 Sep 2017
HGG5NBGX3_n02_ac12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG5NBGX3_n02_ac12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57430023
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2021470.35198836678160483No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1464830.25506345348320686No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1319880.2298240416863493No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1315310.2290282906555688No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC1271060.221323261528208No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1195870.2082308063850157No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC1179990.20546570214676738Illumina Single End PCR Primer 1 (96% over 33bp)
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1142610.1989569114398579No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1094390.19056060625293497No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1084160.18877930799366038No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC973470.16950541705337643No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG917060.1596830285093217No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG836030.14557368364626982No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA808990.14086534494335828No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC785180.13671942983550606No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG764210.1330680295914212No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA730200.1271460399728553No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC730160.12713907497477409No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG724520.12615701024532064No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG707670.12322300480360246No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA674450.11743857389714088No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA671360.11690052779536585No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT669090.11650526415425604No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC663200.1154796681867949No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT660480.11500604831727126No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC660350.11498341207350728No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT648660.11294789138426777No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC643290.11201284039186264No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG638990.11126410309813041No Hit
TGGGTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCG638420.11116485187547287No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG638370.11115614562787132No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT633060.11023154213258804No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC617440.1075117103818677No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG611260.10643561817831765No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC589040.10256656174419433No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA584380.10175513946773102No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA580190.10102555591872216No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC580060.10100291967495817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT182500.072.327681
GGTCTGG233650.064.8727343
TCCAATC204550.064.537562
CCGGCAT82100.053.8853871
CTTGTTA221650.053.333471
TGGGTCT201000.052.170291
ATCAAAG312450.052.052856
CAAAGGA276000.050.524998
CCAATCA282800.049.7004283
TCAAAGG299850.049.6996737
GGGTCTG308200.049.0557062
GGGCACT29950.048.1933251
GTTACGA250000.047.0688864
CGATGGG78200.047.020492
CCTGGCC443250.046.830555
TTACGAC260500.045.273035
TGGCCCG472600.043.7652247
CGCGGAT48350.043.5853041
GAGTCTA99950.042.1984752
TCTGGAG366100.041.6739435
Produced by FastQC (version 0.11.2)