FastQCFastQC Report
Sat 2 Sep 2017
HGG5NBGX3_n01_ct21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG5NBGX3_n01_ct21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85743997
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT13972161.6295204899300413TruSeq Adapter, Index 1 (97% over 36bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA3125800.3645503019879048No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2354060.27454516728442224No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2257540.2632884025688702No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA2215570.2583935992626982TruSeq Adapter, Index 1 (97% over 36bp)
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA2039140.23781723168328625No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1748760.20395130401956885No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1602430.18688538627374696No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1494610.17431074504259464No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1336330.1558511437249654No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1312980.15312792101352588No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1309770.15275355078210315No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1288930.15032305993386336No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1172050.13669178496542445No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT1135040.13237544781123278No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1134770.13234395872634674No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1116690.13023535630138633No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1094570.1276555838655387No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA1075440.12542452388824374No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT1067700.12452183678817771No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT993500.11586816975653699No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC967020.11277990691290027No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG922770.10761919577880186No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG880560.10269640217495342No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT878760.10248647494237995No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC869010.10134936909927351No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA868860.10133187516322571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCC679400.051.8380285
GAGTCTA219800.050.8109362
GGAGTCT223900.050.1052931
GTTACGA252100.048.6139344
CTTGTTA269200.045.7929841
GGGCACT44050.045.7701531
TGGCCCG769400.045.6601647
AGTCTAA157850.043.9749643
GGTCTGG147900.043.03853
CGAAGGA1345250.042.7913868
GTCCAAT198500.042.1517751
CTGGCCC841100.041.9836276
ATCGAAG1391050.041.6551176
CACCTGG856700.041.5213243
TCCAATC203500.041.328682
TTACGAC309450.039.7674675
GAAGGAT1495550.038.5293549
GCTTGTA321450.038.348577
AGTCTAG112300.038.151383
ACCTGGC941650.037.706794
Produced by FastQC (version 0.11.2)