FastQCFastQC Report
Sat 2 Sep 2017
HGG5NBGX3_n01_ac11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG5NBGX3_n01_ac11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23879795
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT312593013.090271503587028TruSeq Adapter, Index 7 (97% over 36bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA649190.27185744266230094No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT602280.25221322042337463No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA566130.2370748995123283TruSeq Adapter, Index 7 (97% over 36bp)
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC499750.20927734094869743No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA457190.19145474238786386No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT452900.18965824455360694No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG424710.1778532855914383No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC386020.16165130395801136No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG381240.15964961173242903No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG356590.14932707755657032No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG337940.14151712776428776No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG325980.13650870955969263No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG325570.13633701629348158TruSeq Adapter, Index 7 (97% over 35bp)
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT307110.1286066316733456No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT298060.1248168168947849No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC295840.12388716067286173No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG289110.12106887852261713No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC288100.12064592681804848No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC286830.1201140964568582No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC285590.11959482901758578No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC265390.11113579492621273No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG260420.10905453752848379No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT249050.10429319012160698No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA246250.10312065074260478No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT245160.10266419791292178TruSeq Adapter, Index 2 (97% over 35bp)
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA241040.10093888996953282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGA55050.091.822771
GTCCAAT49350.074.8515551
TCCAATC59450.061.8933752
GGTCTGG53100.061.2941553
CTTGTTA75750.061.0271341
CCCTCAA37550.059.6383484
CCTCAAC61250.059.486041
GAGCACA8322200.058.670289
AAGAGCA8326550.058.6597
AGAGCAC8332350.058.5611578
ATCAAAG78600.057.0745436
CGGAAGA8716500.056.0798454
GAAGAGC8712550.056.0732776
TCGGAAG8809300.055.4122473
GGAAGAG8845500.055.2465255
GGGCACT9250.054.4912721
GATCGGA8940750.053.6563151
GTTACGA87300.053.283584
TTACGAC88000.052.777915
CCGGCAT21150.052.4301261
Produced by FastQC (version 0.11.2)