FastQCFastQC Report
Thu 22 Sep 2022
HGG3LDRX2_n01_br166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG3LDRX2_n01_br166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9242958
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGCGGCCGCC317930.34396997151777603No Hit
ACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATT315770.34163305729615995No Hit
GTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCTGGAGACTAG266140.2879381254356019No Hit
GTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCT217280.2350762602188607No Hit
GTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAG206640.22356479386793707No Hit
GGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGC166880.18054826171448576No Hit
GTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCCTGGAGACTAG159820.17291001430494438No Hit
GTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCCTGGAGACTAG150690.16303222409968757No Hit
CTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCG120270.1301206821452613No Hit
GTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAG120050.12988266310417076No Hit
GTGCTAGAGCTTGCTACGACCAATTGAGCTATTATGCGCGCGATACGCGT94240.10195870196532321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)