FastQCFastQC Report
Thu 22 Sep 2022
HGG3LDRX2_n01_br135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG3LDRX2_n01_br135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13192159
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGCGGCCGCC500290.37923284581394145No Hit
ACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCTCAATT488100.369992508428681No Hit
GTTCAATACATCGGATCGACGCGTAAACGGTACGTCGGCCTGGAGACTAG412670.3128146044934722No Hit
GTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAGCT366320.2776800977004598No Hit
GTTCAATACATCGGATAGTTCGCGACACCGTTCGTCGGCCTGGAGACTAG329090.24945878836057084No Hit
GGACCAAGCTTCACACCGAGTTCATCGCTTATGTGTTCGACCCTATACGC278340.21098896700684094No Hit
GTTCAATACATCGGACGATACGCGTTGGTACGCGTAAGCCTGGAGACTAG237260.17984925742632424No Hit
CTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCG206520.15654753706349356No Hit
GTTCAATACATCGGACCGTACGTCGTGTCGAACGACGGCCTGGAGACTAG196530.14897485695859186No Hit
GTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATCGCGCGCATAATAG184120.13956775384529552No Hit
GTGCTAGAGCTTGCTACGACCAATTGAGCTATTATGCGCGCGATACGCGT180580.13688434167599103No Hit
GTTCATCGCTTATGTGTTCGACCCTATACGCGGCCGCCGAAACGCGTATC141960.10760937614532996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)