FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatreptsu-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatreptsu-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33517221
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC3635771.0847468529685083TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG456050.064.9565446
GTATGCC469450.063.39318545
CGTATGC476700.062.50981544
CTAATCG477750.062.453233
ATGCCGT475400.062.1874947
ACGTCTG502000.061.01223415
TCGTATG490750.060.47769543
GCACACG514250.059.6077411
TGCCGTC495200.059.46776648
CTCGTAT472000.058.40168842
GCCGTCT500750.058.38230549
CACACGT527500.058.19010512
ACTAATC517100.057.8698832
ACACGTC534850.057.43617213
CACTAAT535650.056.0618231
AGTCACT536900.055.8990428
CACGTCT553050.055.43737414
CGTCTGA552300.055.43035516
AGCACAC558000.055.11105710
GTCACTA544350.055.05008329
Produced by FastQC (version 0.11.2)