FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatreptsu-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatreptsu-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16530720
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1041170.6298394746266345TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG132800.060.56371346
GTATGCC136550.059.3105845
CGTATGC136900.059.18451344
TCGTATG144700.055.99419843
ATGCCGT143400.055.96486347
TGCCGTC143850.054.71925448
CACGTAG155500.054.19890631
GTCACGT157750.053.6253929
TCACGTA158700.053.2602730
CTCGTAT144900.052.84948342
ACGTCTG166100.052.46891815
GCCGTCT148750.051.5520549
AGTCACG166300.051.1208928
GCACACG181550.048.10090311
CACACGT182300.047.82606512
CGTAGAG179500.047.04975533
ACGTAGA179150.047.02445232
ACACGTC187700.046.37555713
CACGTCT191150.045.6112314
CGTCTGA191200.045.59943816
Produced by FastQC (version 0.11.2)