FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatreptsu-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatreptsu-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24710143
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG90050.037.7787615
GCACACG91800.037.02134711
GCGGGGT14050.035.12389450
CACACGT99350.034.2782712
CGGGGTG15750.034.22157751
ATCGGGG32000.031.93696240
ACACGTC113900.029.80725313
CACGTCT122600.027.94842714
AGCACAC130350.026.87908710
CGTCTGA127300.026.69660216
GAGCACA136700.026.0672599
TCGGAAG135200.025.1388533
GATCGGA144700.023.1963021
AATCGGG66500.022.57856839
ATCGGAA155000.021.9938452
CAGTCAC164800.021.152627
TCCAGTC165750.020.77805525
CTGAACT166250.020.75767119
CGGAAGA167400.020.7431624
AATCGCG36050.020.67926239
Produced by FastQC (version 0.11.2)