FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepstw-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepstw-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29216361
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC3459251.1840112463013446TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG428350.065.6680246
CGTATGC439350.064.3106844
GTATGCC446250.063.6221745
ATGCCGT447000.062.43490647
TCGTATG454800.062.18754643
ACGTCTG494200.061.65801615
TGCCGTC451450.061.00545548
GCACACG501750.060.95449411
CACACGT508250.060.11327712
GCCGTCT451000.059.42112749
ACACGTC516400.059.18488313
CTCGTAT450250.059.0461442
ACTAATC457050.058.92585836
CCAACTA511400.058.17944333
CGTCTGA529650.057.59095416
AACTAAT510650.057.32580635
CACGTCT538350.056.6666614
AGCACAC554900.055.3254710
CAACTAA538050.055.15466734
GAGCACA560250.054.7693829
Produced by FastQC (version 0.11.2)