FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepstw-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepstw-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15115136
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC420370.2781119534749803TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC210700.063.85297433
CACCCGT217700.062.23390231
ACCCGTC219300.061.52449432
TCACCCG221850.061.10128430
GTCACCC224550.060.41336429
TATGCCG85750.059.2235146
ACGTCTG241700.058.01031515
CCGTCCA237500.056.0138134
CGTATGC87150.055.86234744
GCACACG252350.055.81192411
GTATGCC93450.055.42960745
CACACGT255200.055.25739312
ACACGTC261700.053.89830413
CACGTCT264150.053.1992914
CGTCTGA263400.053.1914916
ATGCCGT88050.052.3103247
TCGTATG89700.051.11380843
CAGTCAC269150.051.01379827
TCCAGTC275950.050.28957725
AGCACAC283000.049.84247210
Produced by FastQC (version 0.11.2)