FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepstw-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepstw-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24813050
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC7050.012.9076941
CGCGTAT17550.011.1679971
ACGTCTG54400.011.066195515
GCCGAAT37100.011.0376431
CCCCGAT17650.010.9064241
GCCGGTT63900.010.8997531
GCGGACT19650.010.68693351
GCCGGAT51700.010.6285371
GCCAGCT85450.010.4446161
CGGGCCT17150.010.4080761
GCCGATT47300.010.2853261
GCCCGAT20500.010.2438161
GCACACG60800.010.13175911
CGGGCAT36700.010.0135391
CACACGT65350.09.64058912
CGGGGCG2950.00455317749.4914461
CCGGCAT51450.09.3876761
GGGGCGC6151.2774035E-79.10552329
GCCAGTT80350.08.9731661
GCCCGCT16000.08.9686751
Produced by FastQC (version 0.11.2)