FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepst-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepst-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23970479
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC1704610.7111288848253721TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG214450.063.0626246
CGTATGC222750.060.68138544
GTATGCC224600.060.3841445
ATGCCGT229950.058.6291847
TGCCGTC230500.057.71476748
ACGTCTG249900.057.548815
TCGTATG233200.057.54205743
GCACACG260550.055.22416711
GCCGTCT238600.054.6993349
CACACGT268400.053.7265912
CTCGTAT238750.053.66834642
ACACGTC272700.052.94325613
CACGTCT282300.051.09293414
CGTCTGA284600.050.5445416
AGCACAC291750.049.76439310
GAGCACA302100.048.085349
TCTCGTA267350.047.79621541
TCGGAAG310100.046.5495953
CAGTCAC311400.045.58711227
CTGAACT324800.044.63289319
Produced by FastQC (version 0.11.2)