FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepst-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepst-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16383977
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG99100.047.11379615
GCGGGGT21850.046.29197352
CGGGGTG21750.044.412953
GCACACG106150.044.084711
ATCGGGG30350.042.66813742
CACACGT111850.041.99456412
ACACGTC111650.041.85047513
CACGTCT121650.038.55315814
ATGTCAG118500.038.51399634
CGTCTGA123200.038.09640516
ACATGTC123450.037.25319732
AGCACAC127900.037.24603310
GTCAGAA122400.036.9152236
CACATGT129600.035.29635631
TGTCAGA128400.035.24461735
GAGCACA137950.034.8652889
GTCACAT135750.033.92933729
TGCGGGG16850.033.64917451
TCACATG139300.032.98928530
AATCGGG49550.032.27997241
Produced by FastQC (version 0.11.2)