FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepst-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepst-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17960383
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21900.032.7619346
GTCACGC29200.028.64679129
TCACGCC35400.024.123930
CGTATGC31100.023.4079144
CACGCCA38600.021.94264631
GTATGCC34300.021.73427845
ACGTCTG41500.020.74682215
ATGCCGT34750.020.34493347
TCGTATG37950.019.64389243
GCACACG46750.019.01631411
CACACGT49100.017.6784612
TATCTCG46950.016.5492539
ACACGTC53150.016.26547213
TGCCGTC43800.015.82161548
ACGCCAA55700.015.39472132
CGCCAAT57900.014.50753533
CTCGTAT51200.014.28687642
AGTCACG61150.014.25164628
GCCGTCT49300.013.77255449
CGTCTGA65700.013.63763716