FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepshahdara_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepshahdara_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26056358
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT2786431.0693858289788618TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289420.11107461756550935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG314700.064.782748
CGTATGC326950.062.46261646
GTATGCC327200.062.40419447
ATGCCGT334400.061.01858549
ACGTCTG348350.060.37463815
TCGTATG340250.060.0415945
GCACACG354400.059.69077711
TGCCGTC342350.059.3562650
TATCTCG343000.058.52995341
CACACGT361900.058.4826512
GCCGTCT349050.057.85594651
CTCGTAT349650.057.4064344
ACACGTC371500.056.91464213
CACACTG363750.056.83625831
CGTCTGA385500.054.5836716
CACGTCT388850.054.1944314
AGCACAC396900.053.52048510
GAGCACA398950.053.2930229
ACTGATA390300.053.07770534
TGATATA392550.052.8271736
Produced by FastQC (version 0.11.2)