FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepshahdara_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepshahdara_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19454435
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT3472521.7849503210964492TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT855330.43965810366633623TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTT455650.2342139465885285TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTAT301370.1549106926004276TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTTT243330.125076878357043TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGAT220600.11339316716214067TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGAT198760.10216693520012274TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG402900.066.0115248
ACGTCTG858850.064.702115
GTATGCC422750.064.5843447
TCCGTAT826000.064.5414337
GTTCCGT838850.064.4579735
CCGTATC804450.064.4561438
CGTATCT577650.063.96426839
GCACACG870300.063.94885311
TATCGCG164050.063.87593541
CGTATGC396650.063.8662946
TTCCGTA844650.063.83320236
CACACGT873050.063.71903212
ATGCCGT388450.063.5025849
AGTTCCG855950.063.37878434
ACACGTC879350.063.3414813
CGTATCG239600.063.2211139
TATCTCG499550.063.13990841
ACAGTTC864250.063.1183832
CGTCTGA885950.062.77019516
CACGTCT887450.062.61299514
Produced by FastQC (version 0.11.2)