FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepshahdara_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepshahdara_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14831831
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17400.032.78678546
ACGTCTG41950.026.94855115
GCACACG43300.026.43212111
GTATGCC24500.025.28527345
CACACGT48250.024.01051112
CGTATGC24950.023.28616744
ACACGTC51250.022.19518313
ATGCCGT26000.019.9227347
CGTCTGA61750.018.87435516
CACACAG61250.018.57116731
CACAGTG61050.018.230733
CACGTCT63300.018.1358514
TCGTATG29200.017.61955543
AGCACAC67800.017.44913110
ACACAGT65250.017.32542832
GAGCACA71700.016.745239
TGCCGTC32650.016.61533248
TCGGAAG79250.014.6194713
CTGAACT86900.013.6534819
TCCAGTC87500.013.599925
Produced by FastQC (version 0.11.2)