FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepoy-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepoy-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24373106
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC940140.3857284336268016TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG298000.059.1240915
TATGCCG134650.058.0418146
GCACACG308550.057.47801611
ACGGTAG304300.056.7026832
AGTCACG307150.056.27955628
CACACGT315350.056.11651212
GTATGCC146450.055.68332745
GTCACGG311250.055.44790629
CGTATGC144800.054.86758844
ACACGTC323700.054.5283913
CGTCTGA335400.052.5522216
GGTAGCA326250.052.3945934
CACGTCT337600.052.20975514
AGCACAC345800.051.54009210
TCGTATG156750.051.06427843
GAGCACA356350.050.1570669
ATGCCGT143200.049.12602647
TCGGAAG363600.048.809783
GTAGCAT344950.048.34683235
CGGTAGC357550.048.25823233
Produced by FastQC (version 0.11.2)