FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepoy-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepoy-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11919748
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT6460885.420315932853614TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT149890.12574930275371593TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG721450.067.28232648
GTATGCC732600.066.9032647
CGTATGC731750.066.89966646
ATGCCGT723950.066.605249
TCGTATG736500.066.4919645
ACGTCTG795850.066.2444715
CTCGTAT718500.066.2287444
TGCCGTC714950.066.21001450
GCCGTCT695450.065.9880251
GCACACG806900.065.5205711
CACACGT808350.065.36407512
CACAGTC798350.065.20296531
ACAGTCA800400.065.0447132
ACACGTC812400.065.0247513
CACGTCT812350.064.8989514
TCTCGTA736500.064.8144543
CGTCTGA814200.064.72085616
GTCACAG810800.064.3355729
CAGTCAC815450.064.2047727
TCCAGTC816800.064.0215225
Produced by FastQC (version 0.11.2)