FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepoy-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepoy-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15164660
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC171140.11285449195695782TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29500.044.25332646
GTATGCC37200.035.9401245
CGTATGC38050.034.9532944
ACGTCTG47200.031.29296515
ATGCCGT42150.030.63993847
TCGTATG45350.029.71272743
TGCCGTC43850.029.13280748
GCACACG51650.028.0551111
CTCGTAT48700.026.01614842
CACTGAC56050.025.8514731
GCCGTCT47150.025.75768749
ACTGACC56050.025.60169832
CACACGT58650.024.94538712
ACACGTC61200.023.73450313
CACGTCT67750.022.00779514
AGTCACT70350.020.9946628
CGTCTGA70650.020.75764816
AGCACAC72300.020.62347810
GTCACTG77050.018.9873429
GAGCACA84200.018.54159
Produced by FastQC (version 0.11.2)