FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepn13_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepn13_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23364894
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1921800.8225160362379559TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT299500.064.6937334
TATGCCG205650.063.633848
CCGTACG305050.063.55113633
GTACGTA308500.063.01033835
TACGTAA317200.060.9294336
CGTATGC219550.060.84849546
GTATGCC225150.060.2677547
ACGTCTG331300.060.1118815
GCACACG334100.059.67197811
ACCGTAC326500.059.3653132
ATGCCGT212900.059.1324549
CACACGT340850.058.4902712
ACGTAAT332600.057.65579237
TCGTATG234200.057.5802145
ACACGTC347500.057.43126713
TGCCGTC216450.055.7371650
CGTCTGA363400.054.90788316
CTCGTAT253500.054.65987444
CACGTCT366400.054.36301414
AGCACAC372000.053.79110
Produced by FastQC (version 0.11.2)