FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepn13_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepn13_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12019763
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2149921.788654235528604TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC763120.6348877261556655TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG330100.066.63779446
CGTATGC339300.065.9965544
GTATGCC343050.065.9586945
ATGCCGT317950.064.9572547
ACGTCTG498900.064.7662215
TGCCGTC307450.064.35248648
TCGTATG352950.064.3366643
GCACACG504850.064.1010411
CACACGT505250.064.0228412
ACACGTC508550.063.6002513
GTCACCT501300.063.38042429
CGTCTGA514000.062.8706316
CTCGTAT276250.062.68855742
CACGTCT516450.062.55856314
GCCGTCT296300.062.3563849
AGCACAC528000.061.37085710
AGTCACC523350.060.99762728
GAGCACA533150.060.9136479
CAGTCAC525450.060.9006327
TCCAGTC525950.060.86935425
Produced by FastQC (version 0.11.2)