FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepn13_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepn13_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14339556
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43000.023.19776515
ACTTAGG44050.022.80332432
GCACACG45450.022.6405111
TATGCCG16300.021.25730746
CACACGT49800.020.87364812
CACTTAG48300.020.72435831
TCACTTA51500.019.43669730
ACACGTC54200.018.9853813
CGTATGC18650.018.76643644
TTAGGCA54650.018.70050434
TAGGCAT54750.018.02709235
GTATGCC21200.017.99497645
CACGTCT62150.016.61309614
AGTCACT63350.016.24298728
CGTCTGA64450.015.96592816
CTTAGGC66800.015.61350533
GTCACTT67650.015.05528129
AGCACAC71700.014.8886910
GAGCACA76500.014.4587339
TCGTATG26600.013.42082443
Produced by FastQC (version 0.11.2)