FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepmt-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepmt-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25319377
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2159120.8527539994368739TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG244400.063.09646248
CGTGGCC359750.062.29374333
GTATGCC257700.061.48338747
CGTATGC253450.060.98155246
ACGTGGC369350.060.87362332
GCCTTAT362450.060.83497237
ACGTCTG381200.060.64373415
CACGTGG377200.059.8666931
GTCACGT379550.059.54224829
GCACACG391850.059.09446311
TCACGTG385000.058.67210430
CACACGT395850.058.52408212
ATGCCGT253400.058.21738449
GGCCTTA385600.058.05401636
AGTCACG391850.057.95929328
GTGGCCT387650.057.81032634
ACACGTC401750.057.64695713
TCGTATG260100.057.4577245
TATCTCG302000.057.03051441
TGCCGTC254850.056.1008150
Produced by FastQC (version 0.11.2)