FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepmt-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepmt-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20974866
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271240.12931667835208102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG303050.058.5547615
TATCGGG132150.058.10730439
TATCGCG69750.056.85215839
GCACACG315100.056.4609911
CACACGT317550.055.8600512
ACACGTC320100.055.4911913
CACTAGC316900.055.36518531
ACTAGCT318350.054.9700932
AGTCACT323000.054.68839328
CACGTCT327250.054.29965614
TCACTAG325350.053.9919130
GCGGGGT42650.053.9147550
TAGCTTA323600.053.75366634
CGTCTGA331400.053.63011616
CTTATCG223600.053.61052337
ATCGGGG87150.053.13170240
GCTTATC311250.052.9180536
AGCACAC338200.052.761310
CGGGGTG48300.052.5354151
GTCACTA336050.052.42900529
Produced by FastQC (version 0.11.2)