FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepmt-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepmt-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17915743
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG47100.019.09771515
GCACACG51350.017.65355311
TATCGCG11700.017.64932439
CCGATGT50400.017.08309433
CACACGT54800.016.73380712
ACACGTC58600.015.76804713
TATGCCG14800.014.66191446
GATGTAT62600.014.08921835
CACGTCT70100.013.28106214
CGATGTA68700.013.1439234
CGTCTGA72300.012.82856116
ACCGATG71500.012.77604432
AGCACAC75950.012.39672710
GCCGGTT41250.011.87865451
GAGCACA86300.011.5191499
TGTATCG37000.011.35119137
ATGTATC79000.011.16439836
GTATCGC22600.011.15028638
GTATGCC23300.010.815268545
GCCGAAT24000.010.6457131
Produced by FastQC (version 0.11.2)