FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepmr-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepmr-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21205170
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2383781.1241503840808633TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC300750.065.83285533
TATGCCG282600.064.9830548
GTCCGCA306750.064.3968534
CCGCACA306500.063.387436
GTATGCC294000.063.0228147
TCCGCAC313100.063.0013935
CGTATGC291850.062.9354446
ACGTCCG315650.062.8361632
ATGCCGT294400.062.10499249
CGCACAT311200.061.507737
TCGTATG300300.060.87316545
ACGTCTG337650.060.60880715
GTCACGT332100.059.95567329
TGCCGTC302700.059.34990350
CTCGTAT304150.059.13644
GCACACG348200.058.9445611
GCCGTCT293950.058.75906451
AGTCACG342450.058.3582428
CACACGT354200.057.9658312
TCACGTC346200.057.5239130
Produced by FastQC (version 0.11.2)