FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepmr-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepmr-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22206430
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC16097087.248837386288566TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC418840.18861203714419653TruSeq Adapter, Index 9 (98% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267890.12063623013694681No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATGC261680.11783974281323023TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1867650.067.8401446
GTATGCC1876850.067.544945
CGTATGC1878900.067.48237644
ATGCCGT1876500.067.3467347
TCGTATG1884300.067.16453643
ACGTCTG1930100.067.0648215
TGCCGTC1877550.067.0480948
GCCGTCT1867500.066.8260649
GCACACG1939700.066.8098811
CTCGTAT1810300.066.7336842
AGTCACG1923850.066.6589928
GTCACGA1921700.066.6224729
CACACGT1946050.066.5635512
ACACGTC1949250.066.4792713
CGATCAG1923900.066.4207633
TCGGAAG1957400.066.322133
CACGTCT1957450.066.138514
TCACGAT1936400.066.0859830
AGCACAC1962650.066.0637310
CGTCTGA1966750.065.8008416
Produced by FastQC (version 0.11.2)