FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_nutrinetatrepmr-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_nutrinetatrepmr-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26719559
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2590360.9694621082630892TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG318850.064.9604946
GTATGCC331650.062.7803845
CGTATGC332650.062.6863444
ATGCCGT335350.061.54511347
TCGTATG343400.060.57108743
TGCCGTC347600.058.95326648
CTCGTAT335600.057.92204342
GCCGTCT352250.057.17150549
ACGTCTG401550.053.343115
TCACGAT384550.052.76988635
TCTCGTA365800.052.42247841
GTCACAT423300.050.254229
CACGATC381700.049.68871336
CAGTCAC430300.049.68106527
ACACGTC434450.049.40900813
ATCACGA423300.049.35305834
ATCTCGT386850.048.91856840
CACACGT438350.048.91361212
GCACACG439700.048.81101211
CACGTCT439550.048.72351514
Produced by FastQC (version 0.11.2)