FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19175151
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300301.1996254944745937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG58350.044.517215
ACGAGAT119550.024.3116657
TGTACTC40700.022.8969082
TTGTACT42650.020.3304461
GTACTCT45800.019.3106843
TACGAGA138950.018.2206636
CGGCATA164250.017.4478991
TCTAGTT60300.016.7077267
GTAGATA115700.016.474907145
CCCCTGA48000.016.193524
GATCCTG45850.015.5085965
CGGGAAC86150.015.0120951
CGAGGGA65550.014.5960668
CGTGTAT71600.013.867818145
GAACGTA109200.013.7038734
GGAACGT95050.013.6804284
TACGAGG27550.013.4290696
ATCCCCT59900.013.1076392
TACTCTA76350.013.0349774
CCCTGAG75200.012.1573385
Produced by FastQC (version 0.11.5)