FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_Neg.11.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_Neg.11.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11696061
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC352480.3013664172921123Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT216540.1851392532921981No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC137050.11717620145790963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA49850.051.91852145
TAGTTGT128150.042.0835469
CTAGTTG128950.041.709328
GTAGATA170600.040.75293145
TCTAGTT138550.039.4459427
TTGTACT139200.038.497081
ACTCTAG149700.038.3496485
GTACTCT143500.038.089013
CTCTAGT154150.037.2892726
TACTCTA150400.036.9179044
TGTACTC146900.036.5858042
GGATATC53400.031.2394072
CGTGTAT84100.029.740095145
GATATCT61000.028.9961473
ACGTGTT44150.027.422373145
CGGATAT64800.025.4367451
TATCTTG59700.024.1622125
AGCATTA121600.023.5461354
GTACGTT35600.023.418932145
GCATTAG122750.023.384695
Produced by FastQC (version 0.11.5)