FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_Neg.11.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_Neg.11.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9435699
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC159110.16862555704670104Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT119700.12685864608440775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA36150.042.515488145
ACGTGTT56200.037.409447145
TAGTTGT76750.036.738149
TCTAGTT83450.036.1341257
GTAGATA138250.032.77438145
TTGTACT86550.032.5495871
TGTACTC89000.031.8654822
CTAGTTG87750.031.7207788
TACTCTA93300.031.2328114
GTACTCT91800.031.1116123
ACTCTAG115000.026.9780355
CTCTAGT118450.026.62056
AGCATTA73400.026.56584
TCTCGGG66400.026.422073145
GGATATC43150.025.2141442
GATATCT45200.023.414423
CGTGTAT64000.023.33495145
CGGTGGG81550.022.75808145
TCGTGTT89700.022.549212145
TATCTTG40200.021.8184385
Produced by FastQC (version 0.11.5)