FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_8724.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_8724.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13255186
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG166180.12536979865842698No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC160340.12096397591101324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATT51350.049.6899576
AGTGTAT28750.049.676445145
CTATTAG67800.042.2304428
TCGATCT56750.040.878793
CGATCTA59250.040.6186944
AGATTAT101750.039.11651145
ATTAGTT39500.037.441666145
TGTACTC25050.029.2460272
TTGTACT24550.028.9851571
TGTTAAA48800.027.929308145
GATCCTG75450.027.4778675
TATTAGT109650.026.5745459
CTCGATC96100.024.7573242
TCGTGTT47600.024.673468145
GTACGGT34300.024.5154634
TCTATTA109050.024.1283557
GATCTAT109550.023.887525
AACGTAT289800.023.262216
TGTCCTA51250.022.9138155
GTATTCA296300.022.7998859
Produced by FastQC (version 0.11.5)