FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_8404.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_8404.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14456653
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT253850.17559389438205372No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC217300.15031141717242572No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT177070.12248339916576818No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC174210.12050507126372889No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT166990.11551083089564368No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT161190.11149883724815143No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC160060.1107171902099331No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG151520.10480987542552207No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG147120.10176629403776932No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA145940.10095006084741745No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT144720.10010615873535873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA15200.048.171814145
TGTGTAT26500.047.601273145
TCGTGTT46050.043.450485145
GAGTCTA62000.041.2960552
CGCTCAG43100.041.2062844
TACTTCC90600.040.8843235
CTTTTAC94100.040.432771
GCTCAGA46100.040.4106945
TTACTTC93100.039.6317024
ACTTCCT96900.038.5261046
GCCAGTA44800.038.5100024
CTTGTTA37400.036.1104771
TGTCTTG40550.035.935226145
CTCGGCT57400.035.292641
TTACGAC39750.034.1011165
TCCTCTA108950.033.9982269
GTAGCAT51300.033.771298
TTTTACT113000.033.5698052
TTCGCCG81200.033.480385145
TAGCATA51600.033.4341169