FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_8337.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_8337.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16296290
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG176990.10860754196200485No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG172480.10584004089274307No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC168790.10357572183607436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATT47550.069.3716145
GATCCTG54950.038.6515965
TGTGCCG91150.037.381954145
CGCTCAG28100.035.341564
CTATGTT35950.033.072403145
AACGTAT395650.032.4470255
GTATTCA405300.031.6388038
CTCCCTG80750.031.0786672
GAACGTA417600.031.071634
GAAACTA92500.030.7979874
TATTCAC432450.029.7188749
ACGTATT444450.029.1126336
TCGTGTT72300.028.477434145
CGGGAAC352800.028.3376461
CGTATTC462200.027.7427437
GCTCAGA56000.027.312535
GGAACGT376750.026.8811573
CATTGTA205850.026.871658145
AAGAAAC108950.026.7625582
TTGAACG45850.026.5595937
Produced by FastQC (version 0.11.5)